High-resolution structure determination by continuous-rotation data collection in MicroEDED

Brent L. Nannenga, Dan Shi, Andrew G.W. Leslie, Tamir Gonen

Research output: Contribution to journalArticle

102 Scopus citations

Abstract

MicroEDED uses very small three-dimensional protein crystals and electron diffraction for structure determination. We present an improved data collection protocol for MicroEDED called 'continuous rotation'. Microcrystals are continuously rotated during data collection, yielding more accurate data. The method enables data processing with the crystallographic software tool MOSFMOSFMOSFMOSFLM, which resulted in improved resolution for the model protein lysozyme. These improvements are paving the way for the broad implementation and application of MicroEDED in structural biology.

Original languageEnglish (US)
Pages (from-to)927-930
Number of pages4
JournalNature Methods
Volume11
Issue number9
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this