High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography

Sébastien Boutet, Lukas Lomb, Garth J. Williams, Thomas R M Barends, Andrew Aquila, R. Bruce Doak, Uwe Weierstall, Daniel P. DePonte, Jan Steinbrener, Robert L. Shoeman, Marc Messerschmidt, Anton Barty, Thomas A. White, Stephan Kassemeyer, Richard Kirian, M. Marvin Seibert, Paul A. Montanez, Chris Kenney, Ryan Herbst, Philip HartJack Pines, Gunther Haller, Sol M. Gruner, Hugh T. Philipp, Mark W. Tate, Marianne Hromalik, Lucas J. Koerner, Niels Van Bakel, John Morse, Wilfred Ghonsalves, David Arnlund, Michael J. Bogan, Carl Caleman, Raimund Fromme, Christina Y. Hampton, Mark S. Hunter, Linda C. Johansson, Gergely Katona, Christopher Kupitz, Mengning Liang, Andrew V. Martin, Karol Nass, Lars Redecke, Francesco Stellato, Nicusor Timneanu, Dingjie Wang, Nadia Zatsepin, Donald Schafer, James Defever, Richard Neutze, Petra Fromme, John Spence, Henry N. Chapman, Ilme Schlichting

Research output: Contribution to journalArticle

536 Scopus citations

Abstract

Structure determination of proteins and other macromolecules has historically required the growth of high-quality crystals sufficiently large to diffract x-rays efficiently while withstanding radiation damage. We applied serial femtosecond crystallography (SFX) using an x-ray free-electron laser (XFEL) to obtain high-resolution structural information from microcrystals (less than 1 micrometer by 1 micrometer by 3 micrometers) of the well-characterized model protein lysozyme. The agreement with synchrotron data demonstrates the immediate relevance of SFX for analyzing the structure of the large group of difficult-to-crystallize molecules.

Original languageEnglish (US)
Pages (from-to)362-364
Number of pages3
JournalScience
Volume337
Issue number6092
DOIs
StatePublished - Jul 20 2012

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography'. Together they form a unique fingerprint.

  • Cite this

    Boutet, S., Lomb, L., Williams, G. J., Barends, T. R. M., Aquila, A., Doak, R. B., Weierstall, U., DePonte, D. P., Steinbrener, J., Shoeman, R. L., Messerschmidt, M., Barty, A., White, T. A., Kassemeyer, S., Kirian, R., Seibert, M. M., Montanez, P. A., Kenney, C., Herbst, R., ... Schlichting, I. (2012). High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography. Science, 337(6092), 362-364. https://doi.org/10.1126/science.1217737