High mutation rate and predominance of insertions in the Caenohabditis elegans nuclear genome

Dee R. Denver, Krystalynne Morris, Michael Lynch, W. Kelley Thomas

Research output: Contribution to journalArticle

245 Scopus citations

Abstract

Mutations have pivotal functions in the onset of genetic diseases and are the fundamental substrate for evolution. However, present estimates of the spontaneous mutation rate and spectrum are derived from indirect and biased measurements. For instance, mutation rate estimates for Caenorhabditis elegans are extrapolated from observations on a few genetic loci with visible phenotypes and vary over an order of magnitude. Alternative approaches in mammals, relying on phylogenetic comparisons of pseudogene loci and fourfold degenerate codon positions, suffer from uncertainties in the actual number of generations separating the compared species and the inability to exclude biases associated with natural selection. Here we provide a direct and unbiased estimate of the nuclear mutation rate and its molecular spectrum with a set of C. elegans mutation-accumulation lines that reveal a mutation rate about tenfold higher than previous indirect estimates and an excess of insertions over deletions. Because deletions dominate patterns of C. elegans pseudogene variation, our observations indicate that natural selection might be significant in promoting small genome size, and challenge the prevalent assumption that pseudogene divergence accurately reflects the spontaneous mutation spectrum.

Original languageEnglish (US)
Pages (from-to)679-682
Number of pages4
JournalNature
Volume430
Issue number7000
DOIs
StatePublished - Aug 5 2004
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • General

Cite this