High-level generation of polyclonal antibodies by genetic immunization

Ross S. Chambers, Stephen Albert Johnston

Research output: Contribution to journalLetter

73 Scopus citations

Abstract

Antibodies are important tools for investigating the proteome, but current methods for producing them have become a rate-limiting step. A primary obstacle in most methods for generating antibodies or antibody-like molecules is the requirement for at least microgram quantities of purified protein. We have developed a technology for producing antibodies using genetic immunization. Genetic immunization-based antibody production offers several advantages, including high throughput and high specificity. Moreover, antibodies produced from genetically immunized animals are more likely to recognize the native protein. Here we show that a genetic immunization-based system can be used to efficiently raise useful antibodies to a wide range of antigens. We accomplished this by linking the antigen gene to various elements that enhance antigenicity and by codelivering plasmids encoding genetic adjuvants. Our system, which was tested by immunizing mice with <130 antigens, has shown a final success rate of 84%.

Original languageEnglish (US)
Pages (from-to)1088-1092
Number of pages5
JournalNature biotechnology
Volume21
Issue number9
DOIs
StatePublished - Sep 2003
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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