High-efficiency yeast artificial chromosome fragmentation vectors

William J. Pavan; Philip Hieter; Dorothy Sears; Amanda Burkhoff; Roger H. Reeves

doi:10.1016/0378-1119(91)90576-W

High-efficiency yeast artificial chromosome fragmentation vectors

William J. Pavan, Philip Hieter, Dorothy Sears, Amanda Burkhoff, Roger H. Reeves

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Chromosome fragmentation vectors (CFVs) are used to create deletion derivatives of large fragments of human DNA cloned as yeast artificial chromosomes (YACs). CFVs target insertion of a telomere sequence into the YAC via homologous recombination with Alu repetitive elements. This event results in the loss of all YAC sequences distal to the site of integration. A new series of CFVs has been developed. These vectors target fragmentation to both Alu and LINE human repetitive DNA elements. Recovery of deletion derivatives is ten- to 20-fold more efficient with the new vectors than with those described previously.

Original language	English (US)
Pages (from-to)	125-127
Number of pages	3
Journal	Gene
Volume	106
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(91)90576-W
State	Published - Sep 30 1991
Externally published	Yes

Keywords

DNA-mediated transformation
YAC
deletion derivative
homologous recombination
restriction enzyme mapping

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(91)90576-W

Cite this

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title = "High-efficiency yeast artificial chromosome fragmentation vectors",

abstract = "Chromosome fragmentation vectors (CFVs) are used to create deletion derivatives of large fragments of human DNA cloned as yeast artificial chromosomes (YACs). CFVs target insertion of a telomere sequence into the YAC via homologous recombination with Alu repetitive elements. This event results in the loss of all YAC sequences distal to the site of integration. A new series of CFVs has been developed. These vectors target fragmentation to both Alu and LINE human repetitive DNA elements. Recovery of deletion derivatives is ten- to 20-fold more efficient with the new vectors than with those described previously.",

keywords = "DNA-mediated transformation, YAC, deletion derivative, homologous recombination, restriction enzyme mapping",

author = "Pavan, {William J.} and Philip Hieter and Dorothy Sears and Amanda Burkhoff and Reeves, {Roger H.}",

note = "Funding Information: We would like to thank Cheri Byars for excellentt echni-cal assistance, Kurt Runge for the YTCA-lx plasmid and Allan Scott and Steve Mathias for the human LINE containing plasmid. This work was supported by PHS grant HD24605 (P.H. and R.R.).",

year = "1991",

month = sep,

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doi = "10.1016/0378-1119(91)90576-W",

language = "English (US)",

volume = "106",

pages = "125--127",

journal = "Gene",

issn = "0378-1119",

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T1 - High-efficiency yeast artificial chromosome fragmentation vectors

AU - Pavan, William J.

AU - Hieter, Philip

AU - Sears, Dorothy

AU - Burkhoff, Amanda

AU - Reeves, Roger H.

N1 - Funding Information: We would like to thank Cheri Byars for excellentt echni-cal assistance, Kurt Runge for the YTCA-lx plasmid and Allan Scott and Steve Mathias for the human LINE containing plasmid. This work was supported by PHS grant HD24605 (P.H. and R.R.).

PY - 1991/9/30

Y1 - 1991/9/30

N2 - Chromosome fragmentation vectors (CFVs) are used to create deletion derivatives of large fragments of human DNA cloned as yeast artificial chromosomes (YACs). CFVs target insertion of a telomere sequence into the YAC via homologous recombination with Alu repetitive elements. This event results in the loss of all YAC sequences distal to the site of integration. A new series of CFVs has been developed. These vectors target fragmentation to both Alu and LINE human repetitive DNA elements. Recovery of deletion derivatives is ten- to 20-fold more efficient with the new vectors than with those described previously.

AB - Chromosome fragmentation vectors (CFVs) are used to create deletion derivatives of large fragments of human DNA cloned as yeast artificial chromosomes (YACs). CFVs target insertion of a telomere sequence into the YAC via homologous recombination with Alu repetitive elements. This event results in the loss of all YAC sequences distal to the site of integration. A new series of CFVs has been developed. These vectors target fragmentation to both Alu and LINE human repetitive DNA elements. Recovery of deletion derivatives is ten- to 20-fold more efficient with the new vectors than with those described previously.

KW - DNA-mediated transformation

KW - YAC

KW - deletion derivative

KW - homologous recombination

KW - restriction enzyme mapping

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VL - 106

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JO - Gene

JF - Gene

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High-efficiency yeast artificial chromosome fragmentation vectors

Abstract

Keywords

ASJC Scopus subject areas

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