High-efficiency yeast artificial chromosome fragmentation vectors

William J. Pavan; Philip Hieter; Dorothy Sears; Amanda Burkhoff; Roger H. Reeves

doi:10.1016/0378-1119(91)90576-W

High-efficiency yeast artificial chromosome fragmentation vectors

William J. Pavan, Philip Hieter, Dorothy Sears, Amanda Burkhoff, Roger H. Reeves

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Chromosome fragmentation vectors (CFVs) are used to create deletion derivatives of large fragments of human DNA cloned as yeast artificial chromosomes (YACs). CFVs target insertion of a telomere sequence into the YAC via homologous recombination with Alu repetitive elements. This event results in the loss of all YAC sequences distal to the site of integration. A new series of CFVs has been developed. These vectors target fragmentation to both Alu and LINE human repetitive DNA elements. Recovery of deletion derivatives is ten- to 20-fold more efficient with the new vectors than with those described previously.

Original language	English (US)
Pages (from-to)	125-127
Number of pages	3
Journal	Gene
Volume	106
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(91)90576-W
State	Published - Sep 30 1991
Externally published	Yes

Keywords

DNA-mediated transformation
YAC
deletion derivative
homologous recombination
restriction enzyme mapping

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(91)90576-W

Cite this

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title = "High-efficiency yeast artificial chromosome fragmentation vectors",

abstract = "Chromosome fragmentation vectors (CFVs) are used to create deletion derivatives of large fragments of human DNA cloned as yeast artificial chromosomes (YACs). CFVs target insertion of a telomere sequence into the YAC via homologous recombination with Alu repetitive elements. This event results in the loss of all YAC sequences distal to the site of integration. A new series of CFVs has been developed. These vectors target fragmentation to both Alu and LINE human repetitive DNA elements. Recovery of deletion derivatives is ten- to 20-fold more efficient with the new vectors than with those described previously.",

keywords = "DNA-mediated transformation, YAC, deletion derivative, homologous recombination, restriction enzyme mapping",

author = "Pavan, {William J.} and Philip Hieter and Dorothy Sears and Amanda Burkhoff and Reeves, {Roger H.}",

note = "Funding Information: We would like to thank Cheri Byars for excellentt echni-cal assistance, Kurt Runge for the YTCA-lx plasmid and Allan Scott and Steve Mathias for the human LINE containing plasmid. This work was supported by PHS grant HD24605 (P.H. and R.R.).",

year = "1991",

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doi = "10.1016/0378-1119(91)90576-W",

language = "English (US)",

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pages = "125--127",

journal = "Gene",

issn = "0378-1119",

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T1 - High-efficiency yeast artificial chromosome fragmentation vectors

AU - Pavan, William J.

AU - Hieter, Philip

AU - Sears, Dorothy

AU - Burkhoff, Amanda

AU - Reeves, Roger H.

N1 - Funding Information: We would like to thank Cheri Byars for excellentt echni-cal assistance, Kurt Runge for the YTCA-lx plasmid and Allan Scott and Steve Mathias for the human LINE containing plasmid. This work was supported by PHS grant HD24605 (P.H. and R.R.).

PY - 1991/9/30

Y1 - 1991/9/30

N2 - Chromosome fragmentation vectors (CFVs) are used to create deletion derivatives of large fragments of human DNA cloned as yeast artificial chromosomes (YACs). CFVs target insertion of a telomere sequence into the YAC via homologous recombination with Alu repetitive elements. This event results in the loss of all YAC sequences distal to the site of integration. A new series of CFVs has been developed. These vectors target fragmentation to both Alu and LINE human repetitive DNA elements. Recovery of deletion derivatives is ten- to 20-fold more efficient with the new vectors than with those described previously.

AB - Chromosome fragmentation vectors (CFVs) are used to create deletion derivatives of large fragments of human DNA cloned as yeast artificial chromosomes (YACs). CFVs target insertion of a telomere sequence into the YAC via homologous recombination with Alu repetitive elements. This event results in the loss of all YAC sequences distal to the site of integration. A new series of CFVs has been developed. These vectors target fragmentation to both Alu and LINE human repetitive DNA elements. Recovery of deletion derivatives is ten- to 20-fold more efficient with the new vectors than with those described previously.

KW - DNA-mediated transformation

KW - YAC

KW - deletion derivative

KW - homologous recombination

KW - restriction enzyme mapping

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VL - 106

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JO - Gene

JF - Gene

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High-efficiency yeast artificial chromosome fragmentation vectors

Abstract

Keywords

ASJC Scopus subject areas

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