High-efficiency yeast artificial chromosome fragmentation vectors

William J. Pavan, Philip Hieter, Dorothy Sears, Amanda Burkhoff, Roger H. Reeves

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Chromosome fragmentation vectors (CFVs) are used to create deletion derivatives of large fragments of human DNA cloned as yeast artificial chromosomes (YACs). CFVs target insertion of a telomere sequence into the YAC via homologous recombination with Alu repetitive elements. This event results in the loss of all YAC sequences distal to the site of integration. A new series of CFVs has been developed. These vectors target fragmentation to both Alu and LINE human repetitive DNA elements. Recovery of deletion derivatives is ten- to 20-fold more efficient with the new vectors than with those described previously.

Original languageEnglish (US)
Pages (from-to)125-127
Number of pages3
JournalGene
Volume106
Issue number1
DOIs
StatePublished - Sep 30 1991
Externally publishedYes

Keywords

  • DNA-mediated transformation
  • YAC
  • deletion derivative
  • homologous recombination
  • restriction enzyme mapping

ASJC Scopus subject areas

  • Genetics

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    Pavan, W. J., Hieter, P., Sears, D., Burkhoff, A., & Reeves, R. H. (1991). High-efficiency yeast artificial chromosome fragmentation vectors. Gene, 106(1), 125-127. https://doi.org/10.1016/0378-1119(91)90576-W