TY - JOUR
T1 - High Dietary Intervention of Lauric Triglyceride Might be Harmful to Its Improvement of Cholesterol Metabolism in Obese Rats
AU - Xia, Jiaheng
AU - Yu, Ping
AU - Zeng, Zheling
AU - Ma, Maomao
AU - Zhang, Guohua
AU - Wan, Dongman
AU - Gong, Deming
AU - Deng, Shuguang
AU - Wang, Jun
N1 - Funding Information:
This work was supported by the International Science and Technology Cooperation Program of China (project no. 2011DFA32770), the Research Program of State Key Laboratory of Food Science and Technology, Nanchang University (project no. SKLF-ZZB-201517, SKLF-ZZA-201610, SKLF-KF-201812 and SKLF-ZZB-201916), the Science and Technology Program of Jiangxi Province (project no. 20143ACG70015), the National Natural Science Foundation of China (project no. 31701651), and National Natural Science Foundation of China (project no. 32060516).
Publisher Copyright:
© 2021 American Chemical Society.
PY - 2021/4/21
Y1 - 2021/4/21
N2 - Hypercholesterolemia is often considered to be a major risk factor for atherosclerosis, and medium-chain fatty acids have been found to reduce the total cholesterol (TC) level and maintain low-density lipoprotein cholesterol (LDL-c) stability. However, we unexpectedly found that the levels of TC and LDL-c were increased in obese rats treated with high-dose lauric triglycerides (LT). The study aimed to investigate the effect and mechanism of LT on cholesterol metabolism in obese rats. Our results showed that LT intervention could reduce cholesterol biosynthesis by downregulating the expression of HMG-CoA reductase in obese rats. LT increased the expression levels of PPARγ1, LXRα, ABCA1, and ABCG8 in the liver. These results indicated that LT could improve the lipid transfer and bile acid efflux. However, LT significantly increased the expression of PCSK 9, resulting in accelerated degradation of LDLR, thus reducing the transport of very LDL (VLDL) and LDL to the liver. Together with the increased expression of NPC1L1 protein, LT impaired the uptake of VLDL/LDL by the liver and increased the reabsorption of sterols, leading to an increase in the levels of TC and LDL-c in obese rats.
AB - Hypercholesterolemia is often considered to be a major risk factor for atherosclerosis, and medium-chain fatty acids have been found to reduce the total cholesterol (TC) level and maintain low-density lipoprotein cholesterol (LDL-c) stability. However, we unexpectedly found that the levels of TC and LDL-c were increased in obese rats treated with high-dose lauric triglycerides (LT). The study aimed to investigate the effect and mechanism of LT on cholesterol metabolism in obese rats. Our results showed that LT intervention could reduce cholesterol biosynthesis by downregulating the expression of HMG-CoA reductase in obese rats. LT increased the expression levels of PPARγ1, LXRα, ABCA1, and ABCG8 in the liver. These results indicated that LT could improve the lipid transfer and bile acid efflux. However, LT significantly increased the expression of PCSK 9, resulting in accelerated degradation of LDLR, thus reducing the transport of very LDL (VLDL) and LDL to the liver. Together with the increased expression of NPC1L1 protein, LT impaired the uptake of VLDL/LDL by the liver and increased the reabsorption of sterols, leading to an increase in the levels of TC and LDL-c in obese rats.
KW - cholesterol metabolism
KW - lauric triglycerides
KW - low-density lipoprotein receptor
KW - obese rats
KW - reverse cholesterol transport
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U2 - 10.1021/acs.jafc.1c00745
DO - 10.1021/acs.jafc.1c00745
M3 - Article
C2 - 33844520
AN - SCOPUS:85105099570
SN - 0021-8561
VL - 69
SP - 4453
EP - 4463
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 15
ER -