High concentrations of ketocarotenoids in hepatic mitochondria of Haemorhous mexicanus

Zhiyuan Ge, James D. Johnson, Paul A. Cobine, Kevin McGraw, Rosana Garcia, Geoffrey E. Hill

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Vertebrates cannot synthesize carotenoid pigments de novo, so to produce carotenoid-based coloration they must ingest carotenoids. Most songbirds that deposit red carotenoids in feathers, bills, eyes, or skin ingest only yellow or orange dietary pigments, which they oxidize to red pigments via a ketolation reaction. It has been hypothesized that carotenoid ketolation occurs in the liver of vertebrates, but this hypothesis remains to be confirmed. To better understand the role of hepatocytes in the production of ketolated carotenoids in songbirds, we measured the carotenoid content of subcellular components of hepatocytes from wild male house finches (Haemorhous mexicanus) that were molting red, ketocarotenoid-containing feathers (e.g., 3-hydroxy-echinenone). We homogenized freshly collected livers of house finches and isolated subcellular fractions, including mitochondria. We found the highest concentration of ketocarotenoids in the mitochondrial fraction. These observations are consistent with the hypothesis that carotenoid pigments are oxidized on or within hepatic mitochondria, esterified, and then transported to the Golgi apparatus for secretory processing.

Original languageEnglish (US)
Pages (from-to)444-450
Number of pages7
JournalPhysiological and Biochemical Zoology
Volume88
Issue number4
DOIs
StatePublished - Jun 27 2015

Keywords

  • Haemorhous mexicanus
  • Ketocarotenoids
  • Mitochondria

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Physiology
  • Biochemistry

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