This study systematically assessed intracellular electron transfer (IET) and extracellular electron transfer (EET) kinetics with respect to anode potential (Eanode) in a mixed-culture biofilm anode enriched with Geobacter spp. High biofilm conductivity (0.96–1.24 mS cm−1) was maintained during Eanode changes from −0.2 to +0.2 V versus the standard hydrogen electrode (SHE), although the steady-state current density significantly decreased from 2.05 to 0.35 A m−2 in a microbial electrochemical cell. Substantial increase of the Treponema population was observed in the biofilm anode at Eanode=+0.2 V, which reduced intracellular electron-transfer kinetics associated with the maximum specific substrate-utilization rate by a factor of ten. This result suggests that fast EET kinetics can be maintained under dynamic Eanode conditions in a highly conductive biofilm anode as a result of shift of main EET players in the biofilm anode, although Eanode changes can influence IET kinetics.

Original languageEnglish (US)
Pages (from-to)3485-3491
Number of pages7
Issue number24
StatePublished - Dec 20 2016


  • anode potential
  • biofilm
  • conductivity
  • electron transfer
  • microbial electrochemical cell

ASJC Scopus subject areas

  • Environmental Chemistry
  • Chemical Engineering(all)
  • Materials Science(all)
  • Energy(all)

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