TY - JOUR
T1 - Hierarchical Assembly of Nucleic Acid/Coiled-Coil Peptide Nanostructures
AU - Buchberger, Alex
AU - Simmons, Chad R.
AU - Fahmi, Nour Eddine
AU - Freeman, Ronit
AU - Stephanopoulos, Nicholas
N1 - Funding Information:
The authors would like to thank the H. Yan lab for use of their AFM instrument. The authors would like to thank Raghu Pradeep Narayanan for discussions on the DNA origami design and characterization, help with TEM imaging, and providing the M13 scaffold. N.S. acknowledges startup funds from Arizona State University. This material is based upon work supported by the Air Force Office of Scientific Research under Award Number FA9550-17-1-0053.
Publisher Copyright:
Copyright © 2019 American Chemical Society.
PY - 2020/1/22
Y1 - 2020/1/22
N2 - DNA and peptides are two of the most commonly used biomolecules for building self-assembling materials, but few examples exist of hybrid nanostructures that contain both components. Here we report the modification of two peptides that comprise a coiled-coil heterodimer pair with unique DNA handles in order to link DNA origami nanostructures bearing complementary strands into micrometer-long one-dimensional arrays. We probed the effect of number of coils on self-assembly and demonstrated the formation of structures through multiple routes: one-pot assembly, formation of dimers and trimers and an alternating copolymer of two different origami structures, and stepwise assembly of purified structures with coiled-coil conjugates. Our results demonstrate the successful merging of two distinct self-assembly modes to create hybrid bionanomaterials expected to have a range of potential applications in the future.
AB - DNA and peptides are two of the most commonly used biomolecules for building self-assembling materials, but few examples exist of hybrid nanostructures that contain both components. Here we report the modification of two peptides that comprise a coiled-coil heterodimer pair with unique DNA handles in order to link DNA origami nanostructures bearing complementary strands into micrometer-long one-dimensional arrays. We probed the effect of number of coils on self-assembly and demonstrated the formation of structures through multiple routes: one-pot assembly, formation of dimers and trimers and an alternating copolymer of two different origami structures, and stepwise assembly of purified structures with coiled-coil conjugates. Our results demonstrate the successful merging of two distinct self-assembly modes to create hybrid bionanomaterials expected to have a range of potential applications in the future.
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U2 - 10.1021/jacs.9b11158
DO - 10.1021/jacs.9b11158
M3 - Article
C2 - 31820959
AN - SCOPUS:85078527645
SN - 0002-7863
VL - 142
SP - 1406
EP - 1416
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 3
ER -