Heterologous enzyme function in Escherichia coli and the selection of genes encoding the catalytic RNA subunit of RNase P.

N. P. Lawrence, A. Richman, R. Amini, S. Altman

Research output: Contribution to journalArticle

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Abstract

The gene for the catalytic RNA subunit of RNase P has been isolated from several Enterobacteriaceae by complementation of an Escherichia coli strain that is temperature-sensitive for RNase P activity. The selection procedure relies on the ability of the heterologous gene products to function enzymatically in E. coli. This procedure obviates the need for positive results in DNA blot hybridization experiments or for the purification of holoenzyme to identify the RNA component of RNase P and its corresponding gene from organisms other than E. coli. Comparisons of the variations in sequences provide the basis for a refined two-dimensional model of the secondary structure of M1 RNA.

Original languageEnglish (US)
Pages (from-to)6825-6829
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number19
DOIs
StatePublished - Oct 1987

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