Hepatitis C virus quasi-species dynamics predict progression of fibrosis after liver transplantation

Juan I. Arenas, Juan F. Gallegos-Orozco, Tomasz Laskus, Jeff Wilkinson, Amer Khatib, Carlos Fasola, Debra Adair, Marek Radkowski, Karen Kibler, Marek Nowicki, David Douglas, James Williams, George Netto, David Mulligan, Goran Klintmalm, Jorge Rakela, Hugo E. Vargas

Research output: Contribution to journalArticle

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Abstract

Background. The dynamics of hepatitis C virus (HCV) quasi species in the E2 region may correlate with the course of infection after orthotopic liver transplantation (OLT). Methods. Thirty-four patients who underwent transplantation for HCV-related cirrhosis were studied. Serum and liver samples were available before OLT and at 1 week, 4 months, and 1 year after OLT. Patients were divided into group 1 (Knodell/Ishak fibrosis stage [FS] at 1 year, <2) and group 2 (FS at 1 year, ≥2). Complexity was estimated by the number of bands in a single-strand conformational polymorphism assay, whereas diversity was measured by Shannon entropy (SE) and median mobility shift (MMS) values derived from the heteroduplex mobility assay. Diversity dynamics were measured at transmission (before OLT vs. 1 week after OLT) and after OLT (1 week after OLT vs. 1 year after OLT). Results. Complexity was higher in group 1 patients than in group 2 patients before OLT (P < .02) and at 1 week after OLT (P < .04). Diversity decreased in group 1 at transmission, as measured by either SE (P < .01) or MMS (P < .04). However, diversity increased in this group after OLT, as measured by SE (P < .03) or MMS (P < .02). FS at 1 year after OLT correlated with transmission changes, as measured by SE (r = 0.642, P < .0001) and MMS (r = 0.443, P < .04), and with post-OLT changes (for SE: r = -0.583, P < .01; for MMS: r = -0.536, P < .01). Conclusions. HCV complexity and diversity in the E2 region correlated with the severity of recurrence of HCV infection after OLT. Increased diversity of quasi species at transmission correlated with a higher FS at 1 year. However, increased diversity of quasi species in the post-OLT period correlated with a lower FS at 1 year. The dynamics of HCV quasi species in patients who undergo transplantation are predictive of outcome.

Original languageEnglish (US)
Pages (from-to)2037-2046
Number of pages10
JournalJournal of Infectious Diseases
Volume189
Issue number11
DOIs
StatePublished - Apr 1 2004
Externally publishedYes

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Hepacivirus
Liver Transplantation
Fibrosis
Entropy
Transplantation
Virus Diseases

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Arenas, J. I., Gallegos-Orozco, J. F., Laskus, T., Wilkinson, J., Khatib, A., Fasola, C., ... Vargas, H. E. (2004). Hepatitis C virus quasi-species dynamics predict progression of fibrosis after liver transplantation. Journal of Infectious Diseases, 189(11), 2037-2046. https://doi.org/10.1086/386338

Hepatitis C virus quasi-species dynamics predict progression of fibrosis after liver transplantation. / Arenas, Juan I.; Gallegos-Orozco, Juan F.; Laskus, Tomasz; Wilkinson, Jeff; Khatib, Amer; Fasola, Carlos; Adair, Debra; Radkowski, Marek; Kibler, Karen; Nowicki, Marek; Douglas, David; Williams, James; Netto, George; Mulligan, David; Klintmalm, Goran; Rakela, Jorge; Vargas, Hugo E.

In: Journal of Infectious Diseases, Vol. 189, No. 11, 01.04.2004, p. 2037-2046.

Research output: Contribution to journalArticle

Arenas, JI, Gallegos-Orozco, JF, Laskus, T, Wilkinson, J, Khatib, A, Fasola, C, Adair, D, Radkowski, M, Kibler, K, Nowicki, M, Douglas, D, Williams, J, Netto, G, Mulligan, D, Klintmalm, G, Rakela, J & Vargas, HE 2004, 'Hepatitis C virus quasi-species dynamics predict progression of fibrosis after liver transplantation', Journal of Infectious Diseases, vol. 189, no. 11, pp. 2037-2046. https://doi.org/10.1086/386338
Arenas JI, Gallegos-Orozco JF, Laskus T, Wilkinson J, Khatib A, Fasola C et al. Hepatitis C virus quasi-species dynamics predict progression of fibrosis after liver transplantation. Journal of Infectious Diseases. 2004 Apr 1;189(11):2037-2046. https://doi.org/10.1086/386338
Arenas, Juan I. ; Gallegos-Orozco, Juan F. ; Laskus, Tomasz ; Wilkinson, Jeff ; Khatib, Amer ; Fasola, Carlos ; Adair, Debra ; Radkowski, Marek ; Kibler, Karen ; Nowicki, Marek ; Douglas, David ; Williams, James ; Netto, George ; Mulligan, David ; Klintmalm, Goran ; Rakela, Jorge ; Vargas, Hugo E. / Hepatitis C virus quasi-species dynamics predict progression of fibrosis after liver transplantation. In: Journal of Infectious Diseases. 2004 ; Vol. 189, No. 11. pp. 2037-2046.
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abstract = "Background. The dynamics of hepatitis C virus (HCV) quasi species in the E2 region may correlate with the course of infection after orthotopic liver transplantation (OLT). Methods. Thirty-four patients who underwent transplantation for HCV-related cirrhosis were studied. Serum and liver samples were available before OLT and at 1 week, 4 months, and 1 year after OLT. Patients were divided into group 1 (Knodell/Ishak fibrosis stage [FS] at 1 year, <2) and group 2 (FS at 1 year, ≥2). Complexity was estimated by the number of bands in a single-strand conformational polymorphism assay, whereas diversity was measured by Shannon entropy (SE) and median mobility shift (MMS) values derived from the heteroduplex mobility assay. Diversity dynamics were measured at transmission (before OLT vs. 1 week after OLT) and after OLT (1 week after OLT vs. 1 year after OLT). Results. Complexity was higher in group 1 patients than in group 2 patients before OLT (P < .02) and at 1 week after OLT (P < .04). Diversity decreased in group 1 at transmission, as measured by either SE (P < .01) or MMS (P < .04). However, diversity increased in this group after OLT, as measured by SE (P < .03) or MMS (P < .02). FS at 1 year after OLT correlated with transmission changes, as measured by SE (r = 0.642, P < .0001) and MMS (r = 0.443, P < .04), and with post-OLT changes (for SE: r = -0.583, P < .01; for MMS: r = -0.536, P < .01). Conclusions. HCV complexity and diversity in the E2 region correlated with the severity of recurrence of HCV infection after OLT. Increased diversity of quasi species at transmission correlated with a higher FS at 1 year. However, increased diversity of quasi species in the post-OLT period correlated with a lower FS at 1 year. The dynamics of HCV quasi species in patients who undergo transplantation are predictive of outcome.",
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T1 - Hepatitis C virus quasi-species dynamics predict progression of fibrosis after liver transplantation

AU - Arenas, Juan I.

AU - Gallegos-Orozco, Juan F.

AU - Laskus, Tomasz

AU - Wilkinson, Jeff

AU - Khatib, Amer

AU - Fasola, Carlos

AU - Adair, Debra

AU - Radkowski, Marek

AU - Kibler, Karen

AU - Nowicki, Marek

AU - Douglas, David

AU - Williams, James

AU - Netto, George

AU - Mulligan, David

AU - Klintmalm, Goran

AU - Rakela, Jorge

AU - Vargas, Hugo E.

PY - 2004/4/1

Y1 - 2004/4/1

N2 - Background. The dynamics of hepatitis C virus (HCV) quasi species in the E2 region may correlate with the course of infection after orthotopic liver transplantation (OLT). Methods. Thirty-four patients who underwent transplantation for HCV-related cirrhosis were studied. Serum and liver samples were available before OLT and at 1 week, 4 months, and 1 year after OLT. Patients were divided into group 1 (Knodell/Ishak fibrosis stage [FS] at 1 year, <2) and group 2 (FS at 1 year, ≥2). Complexity was estimated by the number of bands in a single-strand conformational polymorphism assay, whereas diversity was measured by Shannon entropy (SE) and median mobility shift (MMS) values derived from the heteroduplex mobility assay. Diversity dynamics were measured at transmission (before OLT vs. 1 week after OLT) and after OLT (1 week after OLT vs. 1 year after OLT). Results. Complexity was higher in group 1 patients than in group 2 patients before OLT (P < .02) and at 1 week after OLT (P < .04). Diversity decreased in group 1 at transmission, as measured by either SE (P < .01) or MMS (P < .04). However, diversity increased in this group after OLT, as measured by SE (P < .03) or MMS (P < .02). FS at 1 year after OLT correlated with transmission changes, as measured by SE (r = 0.642, P < .0001) and MMS (r = 0.443, P < .04), and with post-OLT changes (for SE: r = -0.583, P < .01; for MMS: r = -0.536, P < .01). Conclusions. HCV complexity and diversity in the E2 region correlated with the severity of recurrence of HCV infection after OLT. Increased diversity of quasi species at transmission correlated with a higher FS at 1 year. However, increased diversity of quasi species in the post-OLT period correlated with a lower FS at 1 year. The dynamics of HCV quasi species in patients who undergo transplantation are predictive of outcome.

AB - Background. The dynamics of hepatitis C virus (HCV) quasi species in the E2 region may correlate with the course of infection after orthotopic liver transplantation (OLT). Methods. Thirty-four patients who underwent transplantation for HCV-related cirrhosis were studied. Serum and liver samples were available before OLT and at 1 week, 4 months, and 1 year after OLT. Patients were divided into group 1 (Knodell/Ishak fibrosis stage [FS] at 1 year, <2) and group 2 (FS at 1 year, ≥2). Complexity was estimated by the number of bands in a single-strand conformational polymorphism assay, whereas diversity was measured by Shannon entropy (SE) and median mobility shift (MMS) values derived from the heteroduplex mobility assay. Diversity dynamics were measured at transmission (before OLT vs. 1 week after OLT) and after OLT (1 week after OLT vs. 1 year after OLT). Results. Complexity was higher in group 1 patients than in group 2 patients before OLT (P < .02) and at 1 week after OLT (P < .04). Diversity decreased in group 1 at transmission, as measured by either SE (P < .01) or MMS (P < .04). However, diversity increased in this group after OLT, as measured by SE (P < .03) or MMS (P < .02). FS at 1 year after OLT correlated with transmission changes, as measured by SE (r = 0.642, P < .0001) and MMS (r = 0.443, P < .04), and with post-OLT changes (for SE: r = -0.583, P < .01; for MMS: r = -0.536, P < .01). Conclusions. HCV complexity and diversity in the E2 region correlated with the severity of recurrence of HCV infection after OLT. Increased diversity of quasi species at transmission correlated with a higher FS at 1 year. However, increased diversity of quasi species in the post-OLT period correlated with a lower FS at 1 year. The dynamics of HCV quasi species in patients who undergo transplantation are predictive of outcome.

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