Harbinatic acid, a novel and potent DNA polymerase β inhibitor from Hardwickia binata

Jing Zhen Deng, Shelley R. Starck, Sidney M. Hecht, Carl F. Ijames, Mark E. Hemling

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Bioassay-guided fractionation of an active methyl ethyl ketone extract of Hardwickia binata, using an assay sensitive to DNA polymerase β inhibition, resulted in the isolation of a potent inhibitor. This proved to be a novel diterpenoid, which has been named harbinatic acid (1). The structure of I was established as 3α-O-trans-p-coumaroyl-7-labden-15-oic acid from spectroscopic analysis and by comparison with the published data for a structurally related compound. Compound 1 strongly inhibited calf thymus DNA polymerase β, with an IC50 value of 2.9 μM.

Original languageEnglish (US)
Pages (from-to)1000-1002
Number of pages3
JournalJournal of Natural Products
Volume62
Issue number7
DOIs
StatePublished - Jul 1 1999
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

Cite this