Harbinatic acid, a novel and potent DNA polymerase β inhibitor from Hardwickia binata

Jing Zhen Deng; Shelley R. Starck; Sidney M. Hecht; Carl F. Ijames; Mark E. Hemling

doi:10.1021/np990099r

Harbinatic acid, a novel and potent DNA polymerase β inhibitor from Hardwickia binata

Jing Zhen Deng, Shelley R. Starck, Sidney M. Hecht, Carl F. Ijames, Mark E. Hemling

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Bioassay-guided fractionation of an active methyl ethyl ketone extract of Hardwickia binata, using an assay sensitive to DNA polymerase β inhibition, resulted in the isolation of a potent inhibitor. This proved to be a novel diterpenoid, which has been named harbinatic acid (1). The structure of I was established as 3α-O-trans-p-coumaroyl-7-labden-15-oic acid from spectroscopic analysis and by comparison with the published data for a structurally related compound. Compound 1 strongly inhibited calf thymus DNA polymerase β, with an IC₅₀ value of 2.9 μM.

Original language	English (US)
Pages (from-to)	1000-1002
Number of pages	3
Journal	Journal of Natural Products
Volume	62
Issue number	7
DOIs	https://doi.org/10.1021/np990099r
State	Published - Jul 1999
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
Molecular Medicine
Pharmacology
Pharmaceutical Science
Drug Discovery
Complementary and alternative medicine
Organic Chemistry

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title = "Harbinatic acid, a novel and potent DNA polymerase β inhibitor from Hardwickia binata",

abstract = "Bioassay-guided fractionation of an active methyl ethyl ketone extract of Hardwickia binata, using an assay sensitive to DNA polymerase β inhibition, resulted in the isolation of a potent inhibitor. This proved to be a novel diterpenoid, which has been named harbinatic acid (1). The structure of I was established as 3α-O-trans-p-coumaroyl-7-labden-15-oic acid from spectroscopic analysis and by comparison with the published data for a structurally related compound. Compound 1 strongly inhibited calf thymus DNA polymerase β, with an IC50 value of 2.9 μM.",

author = "Deng, {Jing Zhen} and Starck, {Shelley R.} and Hecht, {Sidney M.} and Ijames, {Carl F.} and Hemling, {Mark E.}",

year = "1999",

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doi = "10.1021/np990099r",

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T1 - Harbinatic acid, a novel and potent DNA polymerase β inhibitor from Hardwickia binata

AU - Deng, Jing Zhen

AU - Starck, Shelley R.

AU - Hecht, Sidney M.

AU - Ijames, Carl F.

AU - Hemling, Mark E.

PY - 1999/7

Y1 - 1999/7

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AB - Bioassay-guided fractionation of an active methyl ethyl ketone extract of Hardwickia binata, using an assay sensitive to DNA polymerase β inhibition, resulted in the isolation of a potent inhibitor. This proved to be a novel diterpenoid, which has been named harbinatic acid (1). The structure of I was established as 3α-O-trans-p-coumaroyl-7-labden-15-oic acid from spectroscopic analysis and by comparison with the published data for a structurally related compound. Compound 1 strongly inhibited calf thymus DNA polymerase β, with an IC50 value of 2.9 μM.

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Harbinatic acid, a novel and potent DNA polymerase β inhibitor from Hardwickia binata

Abstract

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