Harbinatic acid, a novel and potent DNA polymerase β inhibitor from Hardwickia binata

Jing Zhen Deng, Shelley R. Starck, Sidney Hecht, Carl F. Ijames, Mark E. Hemling

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Bioassay-guided fractionation of an active methyl ethyl ketone extract of Hardwickia binata, using an assay sensitive to DNA polymerase β inhibition, resulted in the isolation of a potent inhibitor. This proved to be a novel diterpenoid, which has been named harbinatic acid (1). The structure of I was established as 3α-O-trans-p-coumaroyl-7-labden-15-oic acid from spectroscopic analysis and by comparison with the published data for a structurally related compound. Compound 1 strongly inhibited calf thymus DNA polymerase β, with an IC50 value of 2.9 μM.

Original languageEnglish (US)
Pages (from-to)1000-1002
Number of pages3
JournalJournal of Natural Products
Volume62
Issue number7
DOIs
StatePublished - Jul 1999
Externally publishedYes

Fingerprint

Nucleic Acid Synthesis Inhibitors
DNA-directed DNA polymerase
DNA-Directed DNA Polymerase
Spectroscopic analysis
Bioassay
Diterpenes
acids
Fractionation
ketones
diterpenoids
Biological Assay
Inhibitory Concentration 50
inhibitory concentration 50
Assays
fractionation
bioassays
calves
Acids
extracts
assays

ASJC Scopus subject areas

  • Plant Science
  • Chemistry (miscellaneous)
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Harbinatic acid, a novel and potent DNA polymerase β inhibitor from Hardwickia binata. / Deng, Jing Zhen; Starck, Shelley R.; Hecht, Sidney; Ijames, Carl F.; Hemling, Mark E.

In: Journal of Natural Products, Vol. 62, No. 7, 07.1999, p. 1000-1002.

Research output: Contribution to journalArticle

Deng, Jing Zhen ; Starck, Shelley R. ; Hecht, Sidney ; Ijames, Carl F. ; Hemling, Mark E. / Harbinatic acid, a novel and potent DNA polymerase β inhibitor from Hardwickia binata. In: Journal of Natural Products. 1999 ; Vol. 62, No. 7. pp. 1000-1002.
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