Gut macromolecular permeability in pancreatitis correlates with severity of disease in rats

Colleen M. Ryan, Jan Schmidt, Kent Lewandrowski, Carolyn Compton, David W. Rattner, Andrew L. Warshaw, Ronald G. Tompkins

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Background: Increased intestinal macromolecular permeability could allow absorption of substances from the bowel into the systemic circulation and contribute to multiple organ system failure. Methods: Mild, intermediate, and severe grades of pancreatitis were induced in rats using intravenous caerulein and intraductal glycodeoxycholic acid. [14C]polyethylene glycol (molecular weight, 3350 daltons; 1.1 μCi/142 mg) was instilled into the distal duodenum. At 24 hours, the animals were killed, ascitic fluid was collected for trypsinogen activation peptide measurement, and pancreatic specimens were collected and scored for based on the degree of necrosis, inflammation, and hemorrhage. Results: Gut permeability to polyethylene glycol 3350 (PEG 3350) was increased in animals with early experimental pancreatitis (5.4% ± 1.2%, n = 20) when compared with control animals (1.8% ± 0.2%; n = 6) (P = 0.0005). Furthermore, intestinal macromolecular permeability to PEG 3350 correlated with severity of disease as predicted by the method of induction of pancreatitis (P = 0.0003), the histological findings (P = 0.0002), and total ascitic trypsinogen activation peptides content (P = 0.029). Conclusions: Increased gut permeability in experimental pancreatitis can be correlated with pancreatitis severity.

Original languageEnglish (US)
Pages (from-to)890-895
Number of pages6
JournalGastroenterology
Volume104
Issue number3
StatePublished - 1993
Externally publishedYes

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Pancreatitis
trypsinogen activation peptide
Permeability
Glycodeoxycholic Acid
Ceruletide
Multiple Organ Failure
Ascitic Fluid
Duodenum
Necrosis
Molecular Weight
Hemorrhage
Inflammation
polyethylene glycol 3350

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Ryan, C. M., Schmidt, J., Lewandrowski, K., Compton, C., Rattner, D. W., Warshaw, A. L., & Tompkins, R. G. (1993). Gut macromolecular permeability in pancreatitis correlates with severity of disease in rats. Gastroenterology, 104(3), 890-895.

Gut macromolecular permeability in pancreatitis correlates with severity of disease in rats. / Ryan, Colleen M.; Schmidt, Jan; Lewandrowski, Kent; Compton, Carolyn; Rattner, David W.; Warshaw, Andrew L.; Tompkins, Ronald G.

In: Gastroenterology, Vol. 104, No. 3, 1993, p. 890-895.

Research output: Contribution to journalArticle

Ryan, CM, Schmidt, J, Lewandrowski, K, Compton, C, Rattner, DW, Warshaw, AL & Tompkins, RG 1993, 'Gut macromolecular permeability in pancreatitis correlates with severity of disease in rats', Gastroenterology, vol. 104, no. 3, pp. 890-895.
Ryan CM, Schmidt J, Lewandrowski K, Compton C, Rattner DW, Warshaw AL et al. Gut macromolecular permeability in pancreatitis correlates with severity of disease in rats. Gastroenterology. 1993;104(3):890-895.
Ryan, Colleen M. ; Schmidt, Jan ; Lewandrowski, Kent ; Compton, Carolyn ; Rattner, David W. ; Warshaw, Andrew L. ; Tompkins, Ronald G. / Gut macromolecular permeability in pancreatitis correlates with severity of disease in rats. In: Gastroenterology. 1993 ; Vol. 104, No. 3. pp. 890-895.
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N2 - Background: Increased intestinal macromolecular permeability could allow absorption of substances from the bowel into the systemic circulation and contribute to multiple organ system failure. Methods: Mild, intermediate, and severe grades of pancreatitis were induced in rats using intravenous caerulein and intraductal glycodeoxycholic acid. [14C]polyethylene glycol (molecular weight, 3350 daltons; 1.1 μCi/142 mg) was instilled into the distal duodenum. At 24 hours, the animals were killed, ascitic fluid was collected for trypsinogen activation peptide measurement, and pancreatic specimens were collected and scored for based on the degree of necrosis, inflammation, and hemorrhage. Results: Gut permeability to polyethylene glycol 3350 (PEG 3350) was increased in animals with early experimental pancreatitis (5.4% ± 1.2%, n = 20) when compared with control animals (1.8% ± 0.2%; n = 6) (P = 0.0005). Furthermore, intestinal macromolecular permeability to PEG 3350 correlated with severity of disease as predicted by the method of induction of pancreatitis (P = 0.0003), the histological findings (P = 0.0002), and total ascitic trypsinogen activation peptides content (P = 0.029). Conclusions: Increased gut permeability in experimental pancreatitis can be correlated with pancreatitis severity.

AB - Background: Increased intestinal macromolecular permeability could allow absorption of substances from the bowel into the systemic circulation and contribute to multiple organ system failure. Methods: Mild, intermediate, and severe grades of pancreatitis were induced in rats using intravenous caerulein and intraductal glycodeoxycholic acid. [14C]polyethylene glycol (molecular weight, 3350 daltons; 1.1 μCi/142 mg) was instilled into the distal duodenum. At 24 hours, the animals were killed, ascitic fluid was collected for trypsinogen activation peptide measurement, and pancreatic specimens were collected and scored for based on the degree of necrosis, inflammation, and hemorrhage. Results: Gut permeability to polyethylene glycol 3350 (PEG 3350) was increased in animals with early experimental pancreatitis (5.4% ± 1.2%, n = 20) when compared with control animals (1.8% ± 0.2%; n = 6) (P = 0.0005). Furthermore, intestinal macromolecular permeability to PEG 3350 correlated with severity of disease as predicted by the method of induction of pancreatitis (P = 0.0003), the histological findings (P = 0.0002), and total ascitic trypsinogen activation peptides content (P = 0.029). Conclusions: Increased gut permeability in experimental pancreatitis can be correlated with pancreatitis severity.

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