Guidance channel development: Controlled release of NGF from PLGA scaffolds

Sarah Stabenfeldt, Rebecca Kuntz Willits

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Three categories of stimuli have demonstrated influential effects on neural regeneration: physical, electrical and chemical. Current research is underway to optimize each of these stimuli in a nerve guidance channel in order to achieve neural regeneration at the site of an injured nerve. This project focused on the delivery of nerve growth factor (NGF) from poly(lactic-co-glycolic acid) (PLGA) scaffolds for use in development of nerve guidance channels. More specifically, the main objectives were to fabricate a delivery system to analyze the release of a model protein (ovalbumin) and NGF in an aqueous environment and mathematically model this delivery. Protein-loaded PLGA scaffolds were fabricated by standard dissolution-evaporation techniques with the inclusion of poly(ethylene glycol) (PEG)(MW 8 kDa). Aqueous release profiles of OVA and NGF were determined over a 4-week period through various assay techniques. A MATLAB®(The Mathworks, Inc) program was written to determine the unknown effective diffusion coefficient for the each set of collected data based on an equation derived from Fick's second law. The calculated effective diffusion coefficients for OVA ranged from 7.0 × 10 -9 to 9.3 × 10 -11 cm 2/sec. Results from the NGF release assay illustrated the presence of bioactive NGF.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Pages484-485
Number of pages2
Volume1
Publication statusPublished - 2002
Externally publishedYes
EventProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States
Duration: Oct 23 2002Oct 26 2002

Other

OtherProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS)
CountryUnited States
CityHouston, TX
Period10/23/0210/26/02

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Keywords

  • Controlled release
  • Drug delivery
  • NGF

ASJC Scopus subject areas

  • Bioengineering

Cite this

Stabenfeldt, S., & Willits, R. K. (2002). Guidance channel development: Controlled release of NGF from PLGA scaffolds. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 1, pp. 484-485)