Growth of mammalian cells on substrates coated with cellular microexudates. I. Effect on cell growth at low population densities

L. Weiss, George Poste, A. MacKearnin, K. Willett

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Mammalian and avian cells cultured on glass or plastic substrates produce microexudates of cellular macromolecules which remain bound to the substrate when the cells are detached. The gross macromolecular composition of microexudates from a range of diploid, heteroploid, and virus transformed cells was determined with cells labeled with radioisotopes. Significant differences in the amounts of cellular glycoproteins, proteins, and RNA present in microexudates were found between different cell types and between cells of the same type at different stages of growth. Inoculation of cells onto substrates 'coated' with microexudates altered their growth behavior. Microexudates from exponentially growing subconfluent homotypic and heterotypic cell populations enhanced the growth of mouse and chick embryo cells seeded at very low densities, but similar microexudates had no effect on the proliferation of cells seeded at higher densities. The enhanced growth of low density cell populations seeded on microexudates was compared with the growth enhancement produced by feeder cell layers and conditioned medium.

Original languageEnglish (US)
Pages (from-to)135-145
Number of pages11
JournalJournal of Cell Biology
Volume64
Issue number1
StatePublished - 1975
Externally publishedYes

Fingerprint

Population Density
Growth
Feeder Cells
Population Growth
Chick Embryo
Conditioned Culture Medium
Diploidy
Radioisotopes
Plastics
Glass
Cultured Cells
Glycoproteins
Cell Count
Cell Proliferation
RNA
Viruses

ASJC Scopus subject areas

  • Cell Biology

Cite this

Growth of mammalian cells on substrates coated with cellular microexudates. I. Effect on cell growth at low population densities. / Weiss, L.; Poste, George; MacKearnin, A.; Willett, K.

In: Journal of Cell Biology, Vol. 64, No. 1, 1975, p. 135-145.

Research output: Contribution to journalArticle

@article{bd8509cb619c4479ae4a6d22c4e15c4d,
title = "Growth of mammalian cells on substrates coated with cellular microexudates. I. Effect on cell growth at low population densities",
abstract = "Mammalian and avian cells cultured on glass or plastic substrates produce microexudates of cellular macromolecules which remain bound to the substrate when the cells are detached. The gross macromolecular composition of microexudates from a range of diploid, heteroploid, and virus transformed cells was determined with cells labeled with radioisotopes. Significant differences in the amounts of cellular glycoproteins, proteins, and RNA present in microexudates were found between different cell types and between cells of the same type at different stages of growth. Inoculation of cells onto substrates 'coated' with microexudates altered their growth behavior. Microexudates from exponentially growing subconfluent homotypic and heterotypic cell populations enhanced the growth of mouse and chick embryo cells seeded at very low densities, but similar microexudates had no effect on the proliferation of cells seeded at higher densities. The enhanced growth of low density cell populations seeded on microexudates was compared with the growth enhancement produced by feeder cell layers and conditioned medium.",
author = "L. Weiss and George Poste and A. MacKearnin and K. Willett",
year = "1975",
language = "English (US)",
volume = "64",
pages = "135--145",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "1",

}

TY - JOUR

T1 - Growth of mammalian cells on substrates coated with cellular microexudates. I. Effect on cell growth at low population densities

AU - Weiss, L.

AU - Poste, George

AU - MacKearnin, A.

AU - Willett, K.

PY - 1975

Y1 - 1975

N2 - Mammalian and avian cells cultured on glass or plastic substrates produce microexudates of cellular macromolecules which remain bound to the substrate when the cells are detached. The gross macromolecular composition of microexudates from a range of diploid, heteroploid, and virus transformed cells was determined with cells labeled with radioisotopes. Significant differences in the amounts of cellular glycoproteins, proteins, and RNA present in microexudates were found between different cell types and between cells of the same type at different stages of growth. Inoculation of cells onto substrates 'coated' with microexudates altered their growth behavior. Microexudates from exponentially growing subconfluent homotypic and heterotypic cell populations enhanced the growth of mouse and chick embryo cells seeded at very low densities, but similar microexudates had no effect on the proliferation of cells seeded at higher densities. The enhanced growth of low density cell populations seeded on microexudates was compared with the growth enhancement produced by feeder cell layers and conditioned medium.

AB - Mammalian and avian cells cultured on glass or plastic substrates produce microexudates of cellular macromolecules which remain bound to the substrate when the cells are detached. The gross macromolecular composition of microexudates from a range of diploid, heteroploid, and virus transformed cells was determined with cells labeled with radioisotopes. Significant differences in the amounts of cellular glycoproteins, proteins, and RNA present in microexudates were found between different cell types and between cells of the same type at different stages of growth. Inoculation of cells onto substrates 'coated' with microexudates altered their growth behavior. Microexudates from exponentially growing subconfluent homotypic and heterotypic cell populations enhanced the growth of mouse and chick embryo cells seeded at very low densities, but similar microexudates had no effect on the proliferation of cells seeded at higher densities. The enhanced growth of low density cell populations seeded on microexudates was compared with the growth enhancement produced by feeder cell layers and conditioned medium.

UR - http://www.scopus.com/inward/record.url?scp=0016438806&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016438806&partnerID=8YFLogxK

M3 - Article

C2 - 1167320

AN - SCOPUS:0016438806

VL - 64

SP - 135

EP - 145

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 1

ER -