Abstract

The capacity of Desulfovibrio vulgaris to reduce U(VI) was studied previously with nongrowth conditions involving a high biomass concentration; thus, bacterial growth through respiration of U(VI) was not proven. In this study, we conducted a series of batch tests on U(VI) reduction by D. vulgaris at a low initial biomass (10 to 20 mg/L of protein) that could reveal biomass growth. D. vulgaris grew with U(VI) respiration alone, as well as with simultaneous sulfate reduction. Patterns of growth kinetics and solids production were affected by sulfate and Fe2+. Biogenic sulfide nonenzymatically reduced 76% of the U(VI) and greatly enhanced the overall reduction rate in the absence of Fe2+ but was rapidly scavenged by Fe2+ to form FeS in the presence of Fe2+. Biogenic U solids were uraninite (UO2) nanocrystallites associated with 20 mg/g biomass as protein. The crystallite thickness of UO2 was 4 to 5 nm without Fe2+ but was <1.4 nm in the presence of Fe2+, indicating poor crystallization inhibited by adsorbed Fe2+ and other amorphous Fe solids, such as FeS or FeCO3. This work fills critical gaps in understanding the metabolic utilization of U by microorganisms and formation of UO2 solids in bioremediation sites.

Original languageEnglish (US)
Pages (from-to)6928-6937
Number of pages10
JournalEnvironmental Science and Technology
Volume48
Issue number12
DOIs
StatePublished - Jun 17 2014

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uraninite
Biomass
biomass
Sulfates
respiration
sulfate
Nanocrystallites
protein
Bioremediation
Growth kinetics
Sulfides
Crystallization
bioremediation
Microorganisms
Proteins
crystallization
microorganism
sulfide
kinetics

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Growth of Desulfovibrio vulgaris when respiring U(VI) and characterization of biogenic uraninite. / Zhou, Chen; Vannela, Raveender; Hyun, Sung Pil; Hayes, Kim F.; Rittmann, Bruce.

In: Environmental Science and Technology, Vol. 48, No. 12, 17.06.2014, p. 6928-6937.

Research output: Contribution to journalArticle

Zhou, Chen ; Vannela, Raveender ; Hyun, Sung Pil ; Hayes, Kim F. ; Rittmann, Bruce. / Growth of Desulfovibrio vulgaris when respiring U(VI) and characterization of biogenic uraninite. In: Environmental Science and Technology. 2014 ; Vol. 48, No. 12. pp. 6928-6937.
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