Group-specific 16S rRNA hybridization probes to describe natural communities of methanogens

L. Raskin, J. M. Stromley, Bruce Rittmann, D. A. Stahl

Research output: Contribution to journalArticle

678 Citations (Scopus)

Abstract

Eight oligonucleotides which are complementary to conserved tracts of 16S rRNA from phylogenetically defined groups of methanogens were designed and characterized for use as hybridization probes for studies in environmental and determinative microbiology. The target-group specificity and temperature of dissociation for each probe were characterized. In general, the probes were very specific for the target methanogens and did not hybridize to the rRNAs of nontarget methanogens. Together, the eight probes circumscribe methanogens now represented in pure culture (with the exception of members of the family Methanothermaceae). Three probes are order specific; two identify members of the order Methanobacteriales, and one is specific for the order Methanococcales. The fourth probe encompasses three families belonging to the order Methanomicrobiales, the third order within the current classification. The fifth probe is specific for the remaining family within this order (Methanosarcinaceae). Three additional probes encompass different genera within the Methanosarcinaceae.

Original languageEnglish (US)
Pages (from-to)1232-1240
Number of pages9
JournalApplied and Environmental Microbiology
Volume60
Issue number4
StatePublished - 1994
Externally publishedYes

Fingerprint

methanogens
Methanosarcinaceae
probe
ribosomal RNA
Methanococcales
Methanobacteriales
Methanomicrobiales
Environmental Microbiology
Oligonucleotides
oligonucleotides
microbiology
Temperature
taxonomy
hybridization probes
temperature

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science(all)
  • Microbiology
  • Earth and Planetary Sciences(all)

Cite this

Group-specific 16S rRNA hybridization probes to describe natural communities of methanogens. / Raskin, L.; Stromley, J. M.; Rittmann, Bruce; Stahl, D. A.

In: Applied and Environmental Microbiology, Vol. 60, No. 4, 1994, p. 1232-1240.

Research output: Contribution to journalArticle

@article{8e7ef0ec7b8046aa8f20554677d3c992,
title = "Group-specific 16S rRNA hybridization probes to describe natural communities of methanogens",
abstract = "Eight oligonucleotides which are complementary to conserved tracts of 16S rRNA from phylogenetically defined groups of methanogens were designed and characterized for use as hybridization probes for studies in environmental and determinative microbiology. The target-group specificity and temperature of dissociation for each probe were characterized. In general, the probes were very specific for the target methanogens and did not hybridize to the rRNAs of nontarget methanogens. Together, the eight probes circumscribe methanogens now represented in pure culture (with the exception of members of the family Methanothermaceae). Three probes are order specific; two identify members of the order Methanobacteriales, and one is specific for the order Methanococcales. The fourth probe encompasses three families belonging to the order Methanomicrobiales, the third order within the current classification. The fifth probe is specific for the remaining family within this order (Methanosarcinaceae). Three additional probes encompass different genera within the Methanosarcinaceae.",
author = "L. Raskin and Stromley, {J. M.} and Bruce Rittmann and Stahl, {D. A.}",
year = "1994",
language = "English (US)",
volume = "60",
pages = "1232--1240",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "4",

}

TY - JOUR

T1 - Group-specific 16S rRNA hybridization probes to describe natural communities of methanogens

AU - Raskin, L.

AU - Stromley, J. M.

AU - Rittmann, Bruce

AU - Stahl, D. A.

PY - 1994

Y1 - 1994

N2 - Eight oligonucleotides which are complementary to conserved tracts of 16S rRNA from phylogenetically defined groups of methanogens were designed and characterized for use as hybridization probes for studies in environmental and determinative microbiology. The target-group specificity and temperature of dissociation for each probe were characterized. In general, the probes were very specific for the target methanogens and did not hybridize to the rRNAs of nontarget methanogens. Together, the eight probes circumscribe methanogens now represented in pure culture (with the exception of members of the family Methanothermaceae). Three probes are order specific; two identify members of the order Methanobacteriales, and one is specific for the order Methanococcales. The fourth probe encompasses three families belonging to the order Methanomicrobiales, the third order within the current classification. The fifth probe is specific for the remaining family within this order (Methanosarcinaceae). Three additional probes encompass different genera within the Methanosarcinaceae.

AB - Eight oligonucleotides which are complementary to conserved tracts of 16S rRNA from phylogenetically defined groups of methanogens were designed and characterized for use as hybridization probes for studies in environmental and determinative microbiology. The target-group specificity and temperature of dissociation for each probe were characterized. In general, the probes were very specific for the target methanogens and did not hybridize to the rRNAs of nontarget methanogens. Together, the eight probes circumscribe methanogens now represented in pure culture (with the exception of members of the family Methanothermaceae). Three probes are order specific; two identify members of the order Methanobacteriales, and one is specific for the order Methanococcales. The fourth probe encompasses three families belonging to the order Methanomicrobiales, the third order within the current classification. The fifth probe is specific for the remaining family within this order (Methanosarcinaceae). Three additional probes encompass different genera within the Methanosarcinaceae.

UR - http://www.scopus.com/inward/record.url?scp=0028165830&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028165830&partnerID=8YFLogxK

M3 - Article

C2 - 7517128

AN - SCOPUS:0028165830

VL - 60

SP - 1232

EP - 1240

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 4

ER -