Gross morphological changes in thylakoid membrane structure are associated with photosystem i deletion in Synechocystis sp. PCC 6803

Allison M L Van De Meene, William P. Sharp, Jennifer H. McDaniel, Heiner Friedrich, Willem Vermaas, Robert Roberson

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Cells of Synechocystis sp. PCC 6803 lacking photosystem I (PSI-less) and containing only photosystem II (PSII) or lacking both photosystems I and II (PSI/PSII-less) were compared to wild type (WT) cells to investigate the role of the photosystems in the architecture, structure, and number of thylakoid membranes. All cells were grown at 0.5 μmol photons m- 2 s - 1. The lumen of the thylakoid membranes of the WT cells grown at this low light intensity were inflated compared to cells grown at higher light intensity. Tubular as well as sheet-like thylakoid membranes were found in the PSI-less strain at all stages of development with organized regular arrays of phycobilisomes on the surface of the thylakoid membranes. Tubular structures were also found in the PSI/PSII-less strain, but these were smaller in diameter to those found in the PSI-less strain with what appeared to be a different internal structure and were less common. There were fewer and smaller thylakoid membrane sheets in the double mutant and the phycobilisomes were found on the surface in more disordered arrays. These differences in thylakoid membrane structure most likely reflect the altered composition of photosynthetic particles and distribution of other integral membrane proteins and their interaction with the lipid bilayer. These results suggest an important role for the presence of PSII in the formation of the highly ordered tubular structures.

Original languageEnglish (US)
Pages (from-to)1427-1434
Number of pages8
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1818
Issue number5
DOIs
StatePublished - May 2012

Fingerprint

Synechocystis
Membrane structures
Thylakoids
Photosystem II Protein Complex
Membranes
Photosystem I Protein Complex
Phycobilisomes
Cells
High intensity light
Lipid bilayers
Light
Lipid Bilayers
Photons
Membrane Proteins
Chemical analysis

Keywords

  • Electron tomography
  • Photosynthesis
  • Photosystem I
  • Photosystem II
  • Phycobilisome
  • Thylakoid membrane

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

Cite this

Gross morphological changes in thylakoid membrane structure are associated with photosystem i deletion in Synechocystis sp. PCC 6803. / Van De Meene, Allison M L; Sharp, William P.; McDaniel, Jennifer H.; Friedrich, Heiner; Vermaas, Willem; Roberson, Robert.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1818, No. 5, 05.2012, p. 1427-1434.

Research output: Contribution to journalArticle

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abstract = "Cells of Synechocystis sp. PCC 6803 lacking photosystem I (PSI-less) and containing only photosystem II (PSII) or lacking both photosystems I and II (PSI/PSII-less) were compared to wild type (WT) cells to investigate the role of the photosystems in the architecture, structure, and number of thylakoid membranes. All cells were grown at 0.5 μmol photons m- 2 s - 1. The lumen of the thylakoid membranes of the WT cells grown at this low light intensity were inflated compared to cells grown at higher light intensity. Tubular as well as sheet-like thylakoid membranes were found in the PSI-less strain at all stages of development with organized regular arrays of phycobilisomes on the surface of the thylakoid membranes. Tubular structures were also found in the PSI/PSII-less strain, but these were smaller in diameter to those found in the PSI-less strain with what appeared to be a different internal structure and were less common. There were fewer and smaller thylakoid membrane sheets in the double mutant and the phycobilisomes were found on the surface in more disordered arrays. These differences in thylakoid membrane structure most likely reflect the altered composition of photosynthetic particles and distribution of other integral membrane proteins and their interaction with the lipid bilayer. These results suggest an important role for the presence of PSII in the formation of the highly ordered tubular structures.",
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AB - Cells of Synechocystis sp. PCC 6803 lacking photosystem I (PSI-less) and containing only photosystem II (PSII) or lacking both photosystems I and II (PSI/PSII-less) were compared to wild type (WT) cells to investigate the role of the photosystems in the architecture, structure, and number of thylakoid membranes. All cells were grown at 0.5 μmol photons m- 2 s - 1. The lumen of the thylakoid membranes of the WT cells grown at this low light intensity were inflated compared to cells grown at higher light intensity. Tubular as well as sheet-like thylakoid membranes were found in the PSI-less strain at all stages of development with organized regular arrays of phycobilisomes on the surface of the thylakoid membranes. Tubular structures were also found in the PSI/PSII-less strain, but these were smaller in diameter to those found in the PSI-less strain with what appeared to be a different internal structure and were less common. There were fewer and smaller thylakoid membrane sheets in the double mutant and the phycobilisomes were found on the surface in more disordered arrays. These differences in thylakoid membrane structure most likely reflect the altered composition of photosynthetic particles and distribution of other integral membrane proteins and their interaction with the lipid bilayer. These results suggest an important role for the presence of PSII in the formation of the highly ordered tubular structures.

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