Glycosaminoglycan Binding Properties of the Myxoma Virus CC-chemokine Inhibitor, M-T1

Bruce T. Seet, John Barrett, Janine Robichaud, Brian Shilton, Rajkumari Singh, Douglas McFadden

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Poxviruses encode a number of secreted virulence factors that function to mitigate or modulate the host immune response. M-T1 is a secreted 43-kDa glycoprotein produced by the myxoma virus, a poxvirus pathogen of rabbits, that binds CC-chemokines with high affinity, blocks binding to their cognate G-protein coupled receptors, and thereby inhibits chemokine-induced leukocyte chemotaxis. The present study indicates that M-T1, but not the related vaccinia virus 35-kDa CC-chemokine-binding protein, can localize to cell surfaces through an interaction with glycosaminoglycan molecules. In addition to biochemically characterizing the nature of this interaction, we demonstrate that M-T1 can also simultaneously interact with CC-chemokines while bound to heparin, suggesting that the binding sites on M-T1 for chemokines and heparin are distinct. Furthermore, using recombinant baculovirus-expressed M-T1 truncation and internal deletion mutants, we localize the heparin-binding region of M-T1 to the C terminus of the protein, a region that contains a high abundance of basic residues and includes two clusters of basic amino acid residues that resemble Cardin and Weintraub heparin-binding consensus sequences. The ability of M-T1 to simultaneously interact with chemokines and glycosaminoglycans may enable M-T1 to tether to endothelial surfaces or extracellular matrix and capture host chemokines that are expressed close to sites of virus infection.

Original languageEnglish (US)
Pages (from-to)30504-30513
Number of pages10
JournalJournal of Biological Chemistry
Volume276
Issue number32
DOIs
StatePublished - Aug 10 2001
Externally publishedYes

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Myxoma virus
CC Chemokines
Glycosaminoglycans
Viruses
Chemokines
Heparin
Poxviridae
Leukocyte Chemotaxis
Basic Amino Acids
Vaccinia virus
Baculoviridae
Consensus Sequence
Virulence Factors
Pathogens
Virus Diseases
G-Protein-Coupled Receptors
Protein C
Extracellular Matrix
Glycoproteins
Carrier Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Glycosaminoglycan Binding Properties of the Myxoma Virus CC-chemokine Inhibitor, M-T1. / Seet, Bruce T.; Barrett, John; Robichaud, Janine; Shilton, Brian; Singh, Rajkumari; McFadden, Douglas.

In: Journal of Biological Chemistry, Vol. 276, No. 32, 10.08.2001, p. 30504-30513.

Research output: Contribution to journalArticle

Seet, Bruce T. ; Barrett, John ; Robichaud, Janine ; Shilton, Brian ; Singh, Rajkumari ; McFadden, Douglas. / Glycosaminoglycan Binding Properties of the Myxoma Virus CC-chemokine Inhibitor, M-T1. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 32. pp. 30504-30513.
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