Abstract

Patterns of glucose transporter expression have been well-characterized in mammals. However, data for birds is currently restricted to isolated cells, domestic chickens and chicks, and ducklings. Therefore, in the present study, protein and gene expression of various glucose transporters (GLUTs) in English sparrow extensor digitorum communis, gastrocnemius and pectoralis muscles as well as heart, kidney, and brain tissues were examined. The hypothesis is that the expression pattern of avian GLUTs differs from mammals to maintain the high plasma glucose levels of birds and insulin insensitivity. Our studies failed to identify a GLUT4-like insulin responsive transporter in sparrows. GLUT1 gene expression was identified in all tissues examined and shares 88% homology with chicken and 84% homology with human GLUT1. Compared to the rat control, GLUT1 immunostaining of sparrow extensor digitorum communis muscle was weak and appeared to be localized to blood vessels whereas immunostaining of gastrocnemius muscles was comparable to rat muscle controls. Gene expression of GLUT3 was identified in all tissues examined and shares 90% gene sequence homology with chicken embryonic fibroblast and 75% homology with human GLUT3. Protein expression of GLUT3 was not determined as an avian antibody is not available. Moreover, the C-terminus of the mammalian GLUT3 transporter, against which antibodies are typically designed, differs significantly among species. The predominant difference of chicken and sparrow GLUT expression patterns from that of mammals is the lack of an avian GLUT4. The absence of this insulin responsive GLUT in birds may be a contributing factor to the observed high blood glucose levels and insulin insensitivity.

Original languageEnglish (US)
Pages (from-to)263-270
Number of pages8
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume144
Issue number3
DOIs
StatePublished - Jul 2006

Fingerprint

Sparrows
Facilitative Glucose Transport Proteins
Mammals
Muscle
Chickens
Birds
Gene expression
Insulin
Tissue
Gene Expression
Glucose Transporter Type 3
Skeletal Muscle
Glucose Transporter Type 4
Rat control
Pectoralis Muscles
Muscles
Antibodies
Blood vessels
Fibroblasts
Sequence Homology

Keywords

  • Avian
  • Glucose transporter (GLUT)
  • Immunohistochemistry
  • RT-PCR
  • Sparrow

ASJC Scopus subject areas

  • Biochemistry
  • Physiology

Cite this

Glucose transporter expression in English sparrows (Passer domesticus). / Sweazea, Karen; Braun, Eldon J.

In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 144, No. 3, 07.2006, p. 263-270.

Research output: Contribution to journalArticle

@article{e46264278d9746b6885012be7063601f,
title = "Glucose transporter expression in English sparrows (Passer domesticus)",
abstract = "Patterns of glucose transporter expression have been well-characterized in mammals. However, data for birds is currently restricted to isolated cells, domestic chickens and chicks, and ducklings. Therefore, in the present study, protein and gene expression of various glucose transporters (GLUTs) in English sparrow extensor digitorum communis, gastrocnemius and pectoralis muscles as well as heart, kidney, and brain tissues were examined. The hypothesis is that the expression pattern of avian GLUTs differs from mammals to maintain the high plasma glucose levels of birds and insulin insensitivity. Our studies failed to identify a GLUT4-like insulin responsive transporter in sparrows. GLUT1 gene expression was identified in all tissues examined and shares 88{\%} homology with chicken and 84{\%} homology with human GLUT1. Compared to the rat control, GLUT1 immunostaining of sparrow extensor digitorum communis muscle was weak and appeared to be localized to blood vessels whereas immunostaining of gastrocnemius muscles was comparable to rat muscle controls. Gene expression of GLUT3 was identified in all tissues examined and shares 90{\%} gene sequence homology with chicken embryonic fibroblast and 75{\%} homology with human GLUT3. Protein expression of GLUT3 was not determined as an avian antibody is not available. Moreover, the C-terminus of the mammalian GLUT3 transporter, against which antibodies are typically designed, differs significantly among species. The predominant difference of chicken and sparrow GLUT expression patterns from that of mammals is the lack of an avian GLUT4. The absence of this insulin responsive GLUT in birds may be a contributing factor to the observed high blood glucose levels and insulin insensitivity.",
keywords = "Avian, Glucose transporter (GLUT), Immunohistochemistry, RT-PCR, Sparrow",
author = "Karen Sweazea and Braun, {Eldon J.}",
year = "2006",
month = "7",
doi = "10.1016/j.cbpb.2005.12.027",
language = "English (US)",
volume = "144",
pages = "263--270",
journal = "Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology",
issn = "1096-4959",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Glucose transporter expression in English sparrows (Passer domesticus)

AU - Sweazea, Karen

AU - Braun, Eldon J.

PY - 2006/7

Y1 - 2006/7

N2 - Patterns of glucose transporter expression have been well-characterized in mammals. However, data for birds is currently restricted to isolated cells, domestic chickens and chicks, and ducklings. Therefore, in the present study, protein and gene expression of various glucose transporters (GLUTs) in English sparrow extensor digitorum communis, gastrocnemius and pectoralis muscles as well as heart, kidney, and brain tissues were examined. The hypothesis is that the expression pattern of avian GLUTs differs from mammals to maintain the high plasma glucose levels of birds and insulin insensitivity. Our studies failed to identify a GLUT4-like insulin responsive transporter in sparrows. GLUT1 gene expression was identified in all tissues examined and shares 88% homology with chicken and 84% homology with human GLUT1. Compared to the rat control, GLUT1 immunostaining of sparrow extensor digitorum communis muscle was weak and appeared to be localized to blood vessels whereas immunostaining of gastrocnemius muscles was comparable to rat muscle controls. Gene expression of GLUT3 was identified in all tissues examined and shares 90% gene sequence homology with chicken embryonic fibroblast and 75% homology with human GLUT3. Protein expression of GLUT3 was not determined as an avian antibody is not available. Moreover, the C-terminus of the mammalian GLUT3 transporter, against which antibodies are typically designed, differs significantly among species. The predominant difference of chicken and sparrow GLUT expression patterns from that of mammals is the lack of an avian GLUT4. The absence of this insulin responsive GLUT in birds may be a contributing factor to the observed high blood glucose levels and insulin insensitivity.

AB - Patterns of glucose transporter expression have been well-characterized in mammals. However, data for birds is currently restricted to isolated cells, domestic chickens and chicks, and ducklings. Therefore, in the present study, protein and gene expression of various glucose transporters (GLUTs) in English sparrow extensor digitorum communis, gastrocnemius and pectoralis muscles as well as heart, kidney, and brain tissues were examined. The hypothesis is that the expression pattern of avian GLUTs differs from mammals to maintain the high plasma glucose levels of birds and insulin insensitivity. Our studies failed to identify a GLUT4-like insulin responsive transporter in sparrows. GLUT1 gene expression was identified in all tissues examined and shares 88% homology with chicken and 84% homology with human GLUT1. Compared to the rat control, GLUT1 immunostaining of sparrow extensor digitorum communis muscle was weak and appeared to be localized to blood vessels whereas immunostaining of gastrocnemius muscles was comparable to rat muscle controls. Gene expression of GLUT3 was identified in all tissues examined and shares 90% gene sequence homology with chicken embryonic fibroblast and 75% homology with human GLUT3. Protein expression of GLUT3 was not determined as an avian antibody is not available. Moreover, the C-terminus of the mammalian GLUT3 transporter, against which antibodies are typically designed, differs significantly among species. The predominant difference of chicken and sparrow GLUT expression patterns from that of mammals is the lack of an avian GLUT4. The absence of this insulin responsive GLUT in birds may be a contributing factor to the observed high blood glucose levels and insulin insensitivity.

KW - Avian

KW - Glucose transporter (GLUT)

KW - Immunohistochemistry

KW - RT-PCR

KW - Sparrow

UR - http://www.scopus.com/inward/record.url?scp=33745253132&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745253132&partnerID=8YFLogxK

U2 - 10.1016/j.cbpb.2005.12.027

DO - 10.1016/j.cbpb.2005.12.027

M3 - Article

C2 - 16730206

AN - SCOPUS:33745253132

VL - 144

SP - 263

EP - 270

JO - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology

SN - 1096-4959

IS - 3

ER -