Global expression analysis of two-component system regulator genes during Mycobacterium tuberculosis growth in human macrophages

Shelley E. Haydel; Josephine E. Clark-Curtiss

doi:10.1016/j.femsle.2004.06.010

Global expression analysis of two-component system regulator genes during Mycobacterium tuberculosis growth in human macrophages

Shelley E. Haydel, Josephine E. Clark-Curtiss

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

In the Mycobacterium tuberculosis H37Rv genome, there are 11 paired two-component regulatory system genes, two orphan histidine kinase genes, and six orphan response regulator genes. Expression of the 17 response regulator genes and the two orphan histidine kinase genes during growth of M. tuberculosis in human peripheral blood monocyte-derived macrophages has been analyzed by using cDNA mixtures prepared by the selective capture of transcribed sequences (SCOTS) technique. SCOTS probes were prepared from cDNA obtained from M. tuberculosis grown for 18, 48, and 110 h in human macrophages. Based on expression profiles, the regulatory genes were assigned to three categories: (i) constitutively expressed during growth in macrophages (three genes); (ii) differentially expressed during growth in macrophages (nine genes) and (iii) no detectable expression during growth in macrophages (seven genes).

Original language	English (US)
Pages (from-to)	341-347
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	236
Issue number	2
DOIs	https://doi.org/10.1016/j.femsle.2004.06.010
State	Published - Jul 15 2004
Externally published	Yes

Keywords

Histidine kinase
Intracellular
Macrophage
Mycobacterium tuberculosis
Response regulator
Two-component system

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1016/j.femsle.2004.06.010

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title = "Global expression analysis of two-component system regulator genes during Mycobacterium tuberculosis growth in human macrophages",

abstract = "In the Mycobacterium tuberculosis H37Rv genome, there are 11 paired two-component regulatory system genes, two orphan histidine kinase genes, and six orphan response regulator genes. Expression of the 17 response regulator genes and the two orphan histidine kinase genes during growth of M. tuberculosis in human peripheral blood monocyte-derived macrophages has been analyzed by using cDNA mixtures prepared by the selective capture of transcribed sequences (SCOTS) technique. SCOTS probes were prepared from cDNA obtained from M. tuberculosis grown for 18, 48, and 110 h in human macrophages. Based on expression profiles, the regulatory genes were assigned to three categories: (i) constitutively expressed during growth in macrophages (three genes); (ii) differentially expressed during growth in macrophages (nine genes) and (iii) no detectable expression during growth in macrophages (seven genes).",

keywords = "Histidine kinase, Intracellular, Macrophage, Mycobacterium tuberculosis, Response regulator, Two-component system",

author = "Haydel, {Shelley E.} and Clark-Curtiss, {Josephine E.}",

note = "Funding Information: We thank Roy Curtiss III for helpful discussions and suggestions and for critical review of the manuscript. This work was supported by a Heiser Program of the New York Community Trust fellowship to S.E.H. and NIH grant AI46428 to J.C.C.",

year = "2004",

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doi = "10.1016/j.femsle.2004.06.010",

language = "English (US)",

volume = "236",

pages = "341--347",

journal = "FEMS Microbiology Letters",

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T1 - Global expression analysis of two-component system regulator genes during Mycobacterium tuberculosis growth in human macrophages

AU - Haydel, Shelley E.

AU - Clark-Curtiss, Josephine E.

N1 - Funding Information: We thank Roy Curtiss III for helpful discussions and suggestions and for critical review of the manuscript. This work was supported by a Heiser Program of the New York Community Trust fellowship to S.E.H. and NIH grant AI46428 to J.C.C.

PY - 2004/7/15

Y1 - 2004/7/15

N2 - In the Mycobacterium tuberculosis H37Rv genome, there are 11 paired two-component regulatory system genes, two orphan histidine kinase genes, and six orphan response regulator genes. Expression of the 17 response regulator genes and the two orphan histidine kinase genes during growth of M. tuberculosis in human peripheral blood monocyte-derived macrophages has been analyzed by using cDNA mixtures prepared by the selective capture of transcribed sequences (SCOTS) technique. SCOTS probes were prepared from cDNA obtained from M. tuberculosis grown for 18, 48, and 110 h in human macrophages. Based on expression profiles, the regulatory genes were assigned to three categories: (i) constitutively expressed during growth in macrophages (three genes); (ii) differentially expressed during growth in macrophages (nine genes) and (iii) no detectable expression during growth in macrophages (seven genes).

AB - In the Mycobacterium tuberculosis H37Rv genome, there are 11 paired two-component regulatory system genes, two orphan histidine kinase genes, and six orphan response regulator genes. Expression of the 17 response regulator genes and the two orphan histidine kinase genes during growth of M. tuberculosis in human peripheral blood monocyte-derived macrophages has been analyzed by using cDNA mixtures prepared by the selective capture of transcribed sequences (SCOTS) technique. SCOTS probes were prepared from cDNA obtained from M. tuberculosis grown for 18, 48, and 110 h in human macrophages. Based on expression profiles, the regulatory genes were assigned to three categories: (i) constitutively expressed during growth in macrophages (three genes); (ii) differentially expressed during growth in macrophages (nine genes) and (iii) no detectable expression during growth in macrophages (seven genes).

KW - Histidine kinase

KW - Intracellular

KW - Macrophage

KW - Mycobacterium tuberculosis

KW - Response regulator

KW - Two-component system

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JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2

ER -

Global expression analysis of two-component system regulator genes during Mycobacterium tuberculosis growth in human macrophages

Abstract

Keywords

ASJC Scopus subject areas

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