Global expression analysis of two-component system regulator genes during Mycobacterium tuberculosis growth in human macrophages

Shelley E. Haydel, Josephine E. Clark-Curtiss

Research output: Contribution to journalArticle

52 Scopus citations


In the Mycobacterium tuberculosis H37Rv genome, there are 11 paired two-component regulatory system genes, two orphan histidine kinase genes, and six orphan response regulator genes. Expression of the 17 response regulator genes and the two orphan histidine kinase genes during growth of M. tuberculosis in human peripheral blood monocyte-derived macrophages has been analyzed by using cDNA mixtures prepared by the selective capture of transcribed sequences (SCOTS) technique. SCOTS probes were prepared from cDNA obtained from M. tuberculosis grown for 18, 48, and 110 h in human macrophages. Based on expression profiles, the regulatory genes were assigned to three categories: (i) constitutively expressed during growth in macrophages (three genes); (ii) differentially expressed during growth in macrophages (nine genes) and (iii) no detectable expression during growth in macrophages (seven genes).

Original languageEnglish (US)
Pages (from-to)341-347
Number of pages7
JournalFEMS Microbiology Letters
Issue number2
StatePublished - Jul 15 2004



  • Histidine kinase
  • Intracellular
  • Macrophage
  • Mycobacterium tuberculosis
  • Response regulator
  • Two-component system

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this