TY - JOUR
T1 - Geochemical-compositional-functional changes in arctic soil microbiomes post land submergence revealed by metagenomics
AU - Wang, Nengfei
AU - Guo, Yudong
AU - Li, Gaoyang
AU - Xia, Yan
AU - Ma, Mingyang
AU - Zang, Jiaye
AU - Ma, Yue
AU - Yin, Xiaofei
AU - Han, Wenbing
AU - Lv, Jinjiang
AU - Cao, Huansheng
N1 - Funding Information:
with these factors. Additionally, the relevance of NO2–-N was supported by genes involved in nitrogen utilization.
Funding Information:
We thank Daniel Roush at Arizona State University for his con-structive comments on and critical reading of the manuscript. This research was supported by the National Key R&D Program of China (2018YFC1406700), National Natural Science Foundation of China (No.41776198), and Basic Scientific Fund for National Public Research Institutes of China (Nos.GY0219Q10).
Publisher Copyright:
© 2019, Japanese Society of Microbial Ecology. All rights reserved.
PY - 2019
Y1 - 2019
N2 - Lakes of meltwater in the Artic have become one of the transforming landscape changes under global warming. We herein compared microbial communities between sediments and bank soils at an arctic lake post land submergence using geochemistry, 16S rRNA amplicons, and metagenomes. The results obtained showed that each sample had approximately 2,609 OTUs on average and shared 1,716 OTUs based on the 16S rRNA gene V3–V4 region. Dominant phyla in sediments and soils included Proteobacteria, Acidobacteria, Actinobacteria, Gemmatimonadetes, and Nitrospirae; sediments contained a unique phylum, Euryarchaeota, with the phylum Thaumarchaeota being primarily present in bank soils. Among the top 35 genera across all sites, 17 were more abundant in sediments, while the remaining 18 were more abundant in bank soils; seven out of the top ten genera across all sites were only from sediments. A redundancy analysis separated sediment samples from soil samples based on the components of nitrite and ammonium. Metagenome results supported the role of nitrite because most of the genes for denitrification and methane metabolic genes were more abundant in sediments than in soils, while the abundance of phosphorus-utilizing genes was similar and, thus, was not a significant explanatory factor. We identified several modules from the global networks of OTUs that were closely related to some geochemical factors, such as pH and nitrite. Collectively, the present results showing consistent changes in geochemistry, microbiome compositions, and functional genes suggest an ecological mechanism across molecular and community levels that structures microbiomes post land submergence.
AB - Lakes of meltwater in the Artic have become one of the transforming landscape changes under global warming. We herein compared microbial communities between sediments and bank soils at an arctic lake post land submergence using geochemistry, 16S rRNA amplicons, and metagenomes. The results obtained showed that each sample had approximately 2,609 OTUs on average and shared 1,716 OTUs based on the 16S rRNA gene V3–V4 region. Dominant phyla in sediments and soils included Proteobacteria, Acidobacteria, Actinobacteria, Gemmatimonadetes, and Nitrospirae; sediments contained a unique phylum, Euryarchaeota, with the phylum Thaumarchaeota being primarily present in bank soils. Among the top 35 genera across all sites, 17 were more abundant in sediments, while the remaining 18 were more abundant in bank soils; seven out of the top ten genera across all sites were only from sediments. A redundancy analysis separated sediment samples from soil samples based on the components of nitrite and ammonium. Metagenome results supported the role of nitrite because most of the genes for denitrification and methane metabolic genes were more abundant in sediments than in soils, while the abundance of phosphorus-utilizing genes was similar and, thus, was not a significant explanatory factor. We identified several modules from the global networks of OTUs that were closely related to some geochemical factors, such as pH and nitrite. Collectively, the present results showing consistent changes in geochemistry, microbiome compositions, and functional genes suggest an ecological mechanism across molecular and community levels that structures microbiomes post land submergence.
KW - 16S rRNA gene
KW - Meltwater
KW - Metagenome
KW - Soil microbiomes
KW - The Arctic
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U2 - 10.1264/jsme2.ME18091
DO - 10.1264/jsme2.ME18091
M3 - Article
C2 - 31178526
AN - SCOPUS:85068638094
SN - 1342-6311
VL - 34
SP - 180
EP - 190
JO - Microbes and Environments
JF - Microbes and Environments
IS - 2
ER -