TY - JOUR
T1 - Genotoxic effects of copper oxide nanoparticles in Neuro 2A cell cultures
AU - Perreault, François
AU - Melegari, Silvia Pedroso
AU - da Costa, Cristina Henning
AU - de Oliveira Franco Rossetto, Ana Letícia
AU - Popovic, Radovan
AU - Matias, William Gerson
N1 - Funding Information:
This work was supported by research grants awarded to W.G. Matias by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brazil), and to R. Popovic by the Natural Sciences and Engineering Research Council (Canada). F. Perreault was supported by a mobility fellowship from the Ministère de l'Éducation, des Loisirs et du Sport du Québec for his work in the LABTOX.
PY - 2012/12/15
Y1 - 2012/12/15
N2 - Copper oxide nanoparticles (CuO NPs) are used for their biocide potential however they were also shown to be highly toxic to mammalian cells. Therefore, the effects of CuO NPs should be carefully investigated to determine the most sensitive processes for CuO NP toxicity. In this study, the genotoxicity of CuO NPs was investigated in vitro, using the mouse neuroblastoma cell line Neuro-2A. Genotoxic effects related to DNA fragmentation, DNA methylation and chromosomal damage, as well as lipid peroxidation, were investigated and compared to cytotoxic effects, measured by the mitochondrial reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide into formazan. Based on mitochondrial activity, CuO NPs were found to be cytotoxic. At the highest concentration tested (400mgl-1), 63% of cell viability was found in Neuro-2A cells after 24h of treatment to CuO NPs. CuO NPs were also found to induce DNA fragmentation, lipid peroxidation and micronucleus formation. The micronucleus assay was the most sensitive to evaluate CuO NP genotoxicity and micronucleus frequency was increased significantly at 12.5mgl-1 CuO NPs after 24h of treatment. At this concentration, no significant change of cell viability was found using the mitochondrial activity assay. These results highlight the important risk of genotoxic effects of CuO NPs and show that genotoxicity assays are a sensitive approach to evaluate the risk of CuO NP toxicity.
AB - Copper oxide nanoparticles (CuO NPs) are used for their biocide potential however they were also shown to be highly toxic to mammalian cells. Therefore, the effects of CuO NPs should be carefully investigated to determine the most sensitive processes for CuO NP toxicity. In this study, the genotoxicity of CuO NPs was investigated in vitro, using the mouse neuroblastoma cell line Neuro-2A. Genotoxic effects related to DNA fragmentation, DNA methylation and chromosomal damage, as well as lipid peroxidation, were investigated and compared to cytotoxic effects, measured by the mitochondrial reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide into formazan. Based on mitochondrial activity, CuO NPs were found to be cytotoxic. At the highest concentration tested (400mgl-1), 63% of cell viability was found in Neuro-2A cells after 24h of treatment to CuO NPs. CuO NPs were also found to induce DNA fragmentation, lipid peroxidation and micronucleus formation. The micronucleus assay was the most sensitive to evaluate CuO NP genotoxicity and micronucleus frequency was increased significantly at 12.5mgl-1 CuO NPs after 24h of treatment. At this concentration, no significant change of cell viability was found using the mitochondrial activity assay. These results highlight the important risk of genotoxic effects of CuO NPs and show that genotoxicity assays are a sensitive approach to evaluate the risk of CuO NP toxicity.
KW - Cytotoxicity
KW - DNA methylation
KW - Lipid peroxidation
KW - Micronucleus
KW - Nanotoxicology
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U2 - 10.1016/j.scitotenv.2012.09.065
DO - 10.1016/j.scitotenv.2012.09.065
M3 - Article
C2 - 23137976
AN - SCOPUS:84868254935
SN - 0048-9697
VL - 441
SP - 117
EP - 124
JO - Science of the Total Environment
JF - Science of the Total Environment
ER -