Genotoxic effects of copper oxide nanoparticles in Neuro 2A cell cultures

Francois Perreault, Silvia Pedroso Melegari, Cristina Henning da Costa, Ana Letícia de Oliveira Franco Rossetto, Radovan Popovic, William Gerson Matias

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Copper oxide nanoparticles (CuO NPs) are used for their biocide potential however they were also shown to be highly toxic to mammalian cells. Therefore, the effects of CuO NPs should be carefully investigated to determine the most sensitive processes for CuO NP toxicity. In this study, the genotoxicity of CuO NPs was investigated in vitro, using the mouse neuroblastoma cell line Neuro-2A. Genotoxic effects related to DNA fragmentation, DNA methylation and chromosomal damage, as well as lipid peroxidation, were investigated and compared to cytotoxic effects, measured by the mitochondrial reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide into formazan. Based on mitochondrial activity, CuO NPs were found to be cytotoxic. At the highest concentration tested (400mgl-1), 63% of cell viability was found in Neuro-2A cells after 24h of treatment to CuO NPs. CuO NPs were also found to induce DNA fragmentation, lipid peroxidation and micronucleus formation. The micronucleus assay was the most sensitive to evaluate CuO NP genotoxicity and micronucleus frequency was increased significantly at 12.5mgl-1 CuO NPs after 24h of treatment. At this concentration, no significant change of cell viability was found using the mitochondrial activity assay. These results highlight the important risk of genotoxic effects of CuO NPs and show that genotoxicity assays are a sensitive approach to evaluate the risk of CuO NP toxicity.

Original languageEnglish (US)
Pages (from-to)117-124
Number of pages8
JournalScience of the Total Environment
Volume441
DOIs
StatePublished - Dec 15 2012
Externally publishedYes

Fingerprint

Copper oxides
Cell culture
genotoxicity
Cells
oxide
Nanoparticles
copper
Assays
assay
DNA
Lipids
Toxicity
fragmentation
viability
lipid
Formazans
toxicity
Biocides
Disinfectants
Poisons

Keywords

  • Cytotoxicity
  • DNA methylation
  • Lipid peroxidation
  • Micronucleus
  • Nanotoxicology

ASJC Scopus subject areas

  • Environmental Chemistry
  • Pollution
  • Waste Management and Disposal
  • Environmental Engineering

Cite this

Perreault, F., Melegari, S. P., da Costa, C. H., de Oliveira Franco Rossetto, A. L., Popovic, R., & Matias, W. G. (2012). Genotoxic effects of copper oxide nanoparticles in Neuro 2A cell cultures. Science of the Total Environment, 441, 117-124. https://doi.org/10.1016/j.scitotenv.2012.09.065

Genotoxic effects of copper oxide nanoparticles in Neuro 2A cell cultures. / Perreault, Francois; Melegari, Silvia Pedroso; da Costa, Cristina Henning; de Oliveira Franco Rossetto, Ana Letícia; Popovic, Radovan; Matias, William Gerson.

In: Science of the Total Environment, Vol. 441, 15.12.2012, p. 117-124.

Research output: Contribution to journalArticle

Perreault, F, Melegari, SP, da Costa, CH, de Oliveira Franco Rossetto, AL, Popovic, R & Matias, WG 2012, 'Genotoxic effects of copper oxide nanoparticles in Neuro 2A cell cultures', Science of the Total Environment, vol. 441, pp. 117-124. https://doi.org/10.1016/j.scitotenv.2012.09.065
Perreault F, Melegari SP, da Costa CH, de Oliveira Franco Rossetto AL, Popovic R, Matias WG. Genotoxic effects of copper oxide nanoparticles in Neuro 2A cell cultures. Science of the Total Environment. 2012 Dec 15;441:117-124. https://doi.org/10.1016/j.scitotenv.2012.09.065
Perreault, Francois ; Melegari, Silvia Pedroso ; da Costa, Cristina Henning ; de Oliveira Franco Rossetto, Ana Letícia ; Popovic, Radovan ; Matias, William Gerson. / Genotoxic effects of copper oxide nanoparticles in Neuro 2A cell cultures. In: Science of the Total Environment. 2012 ; Vol. 441. pp. 117-124.
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