Genomic composition and dynamics among Methanomicrobiales predict adaptation to contrasting environments

Patrick Browne, Hideyuki Tamaki, Nikos Kyrpides, Tanja Woyke, Lynne Goodwin, Hiroyuki Imachi, Suzanna Bräuer, Joseph B. Yavitt, Wen Tso Liu, Stephen Zinder, Hinsby Cadillo-Quiroz

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Members of the order Methanomicrobiales are abundant, and sometimes dominant, hydrogenotrophic (H2-CO2 utilizing) methanoarchaea in a broad range of anoxic habitats. Despite their key roles in greenhouse gas emissions and waste conversion to methane, little is known about the physiological and genomic bases for their widespread distribution and abundance. In this study, we compared the genomes of nine diverse Methanomicrobiales strains, examined their pangenomes, reconstructed gene flow and identified genes putatively mediating their success across different habitats. Most strains slowly increased gene content whereas one, Methanocorpusculum labreanum, evidenced genome downsizing. Peat-dwelling Methanomicrobiales showed adaptations centered on improved transport of scarce inorganic nutrients and likely use H+ rather than Na+ transmembrane chemiosmotic gradients during energy conservation. In contrast, other Methanomicrobiales show the potential to concurrently use Na+ and H+ chemiosmotic gradients. Analyses also revealed that the Methanomicrobiales lack a canonical electron bifurcation system (MvhABGD) known to produce low potential electrons in other orders of hydrogenotrophic methanogens. Additional putative differences in anabolic metabolism suggest that the dynamics of interspecies electron transfer from Methanomicrobiales syntrophic partners can also differ considerably. Altogether, these findings suggest profound differences in electron trafficking in the Methanomicrobiales compared with other hydrogenotrophs, and warrant further functional evaluations.The ISME Journal advance online publication, 23 August 2016; doi:10.1038/ismej.2016.104.

Original languageEnglish (US)
JournalISME Journal
DOIs
StateAccepted/In press - Aug 23 2016

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Methanomicrobiales
genomics
electron
Electrons
genome
electrons
trafficking
gene
habitat
energy conservation
bifurcation
Ecosystem
gene flow
peat
Genome
greenhouse gas
metabolism
methane
methanogens
Gene Flow

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Genomic composition and dynamics among Methanomicrobiales predict adaptation to contrasting environments. / Browne, Patrick; Tamaki, Hideyuki; Kyrpides, Nikos; Woyke, Tanja; Goodwin, Lynne; Imachi, Hiroyuki; Bräuer, Suzanna; Yavitt, Joseph B.; Liu, Wen Tso; Zinder, Stephen; Cadillo-Quiroz, Hinsby.

In: ISME Journal, 23.08.2016.

Research output: Contribution to journalArticle

Browne, P, Tamaki, H, Kyrpides, N, Woyke, T, Goodwin, L, Imachi, H, Bräuer, S, Yavitt, JB, Liu, WT, Zinder, S & Cadillo-Quiroz, H 2016, 'Genomic composition and dynamics among Methanomicrobiales predict adaptation to contrasting environments', ISME Journal. https://doi.org/10.1038/ismej.2016.104
Browne, Patrick ; Tamaki, Hideyuki ; Kyrpides, Nikos ; Woyke, Tanja ; Goodwin, Lynne ; Imachi, Hiroyuki ; Bräuer, Suzanna ; Yavitt, Joseph B. ; Liu, Wen Tso ; Zinder, Stephen ; Cadillo-Quiroz, Hinsby. / Genomic composition and dynamics among Methanomicrobiales predict adaptation to contrasting environments. In: ISME Journal. 2016.
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