Genomic and functional evolution of the Drosophila melanogaster sperm proteome

Steve Dorus, Scott A. Busby, Ursula Gerike, Jeffrey Shabanowitz, Donald F. Hunt, Timothy L. Karr

Research output: Contribution to journalArticle

188 Citations (Scopus)

Abstract

In addition to delivering a haploid genome to the egg, sperm have additional critical functions, including egg activation, origination of the zygote centrosome and delivery of paternal factors. Despite this, existing knowledge of the molecular basis of sperm form and function is limited. We used whole-sperm mass spectrometry to identify 381 proteins of the Drosophila melanogaster sperm proteome (DmSP). This approach identified mitochondrial, metabolic and cytoskeletal proteins, in addition to several new functional categories. We also observed nonrandom genomic clustering of sperm genes and underrepresentation on the X chromosome. Identification of widespread functional constraint on the proteome indicates that sexual selection has had a limited role in the overall evolution of D. melanogaster sperm. The relevance of the DmSP to the study of mammalian sperm function and fertilization mechanisms is demonstrated by the identification of substantial homology between the DmSP and proteins of the mouse axoneme accessory structure.

Original languageEnglish (US)
Pages (from-to)1440-1445
Number of pages6
JournalNature Genetics
Volume38
Issue number12
DOIs
StatePublished - Dec 5 2006
Externally publishedYes

Fingerprint

Proteome
Drosophila melanogaster
Spermatozoa
Ovum
Axoneme
Drosophila Proteins
Centrosome
Cytoskeletal Proteins
Zygote
Haploidy
Mitochondrial Proteins
X Chromosome
Fertilization
Cluster Analysis
Mass Spectrometry
Genome

ASJC Scopus subject areas

  • Genetics

Cite this

Dorus, S., Busby, S. A., Gerike, U., Shabanowitz, J., Hunt, D. F., & Karr, T. L. (2006). Genomic and functional evolution of the Drosophila melanogaster sperm proteome. Nature Genetics, 38(12), 1440-1445. https://doi.org/10.1038/ng1915

Genomic and functional evolution of the Drosophila melanogaster sperm proteome. / Dorus, Steve; Busby, Scott A.; Gerike, Ursula; Shabanowitz, Jeffrey; Hunt, Donald F.; Karr, Timothy L.

In: Nature Genetics, Vol. 38, No. 12, 05.12.2006, p. 1440-1445.

Research output: Contribution to journalArticle

Dorus, S, Busby, SA, Gerike, U, Shabanowitz, J, Hunt, DF & Karr, TL 2006, 'Genomic and functional evolution of the Drosophila melanogaster sperm proteome', Nature Genetics, vol. 38, no. 12, pp. 1440-1445. https://doi.org/10.1038/ng1915
Dorus, Steve ; Busby, Scott A. ; Gerike, Ursula ; Shabanowitz, Jeffrey ; Hunt, Donald F. ; Karr, Timothy L. / Genomic and functional evolution of the Drosophila melanogaster sperm proteome. In: Nature Genetics. 2006 ; Vol. 38, No. 12. pp. 1440-1445.
@article{b04889e0ad2c40e594ed39f7e8bccc7a,
title = "Genomic and functional evolution of the Drosophila melanogaster sperm proteome",
abstract = "In addition to delivering a haploid genome to the egg, sperm have additional critical functions, including egg activation, origination of the zygote centrosome and delivery of paternal factors. Despite this, existing knowledge of the molecular basis of sperm form and function is limited. We used whole-sperm mass spectrometry to identify 381 proteins of the Drosophila melanogaster sperm proteome (DmSP). This approach identified mitochondrial, metabolic and cytoskeletal proteins, in addition to several new functional categories. We also observed nonrandom genomic clustering of sperm genes and underrepresentation on the X chromosome. Identification of widespread functional constraint on the proteome indicates that sexual selection has had a limited role in the overall evolution of D. melanogaster sperm. The relevance of the DmSP to the study of mammalian sperm function and fertilization mechanisms is demonstrated by the identification of substantial homology between the DmSP and proteins of the mouse axoneme accessory structure.",
author = "Steve Dorus and Busby, {Scott A.} and Ursula Gerike and Jeffrey Shabanowitz and Hunt, {Donald F.} and Karr, {Timothy L.}",
year = "2006",
month = "12",
day = "5",
doi = "10.1038/ng1915",
language = "English (US)",
volume = "38",
pages = "1440--1445",
journal = "Nature Genetics",
issn = "1061-4036",
publisher = "Nature Publishing Group",
number = "12",

}

TY - JOUR

T1 - Genomic and functional evolution of the Drosophila melanogaster sperm proteome

AU - Dorus, Steve

AU - Busby, Scott A.

AU - Gerike, Ursula

AU - Shabanowitz, Jeffrey

AU - Hunt, Donald F.

AU - Karr, Timothy L.

PY - 2006/12/5

Y1 - 2006/12/5

N2 - In addition to delivering a haploid genome to the egg, sperm have additional critical functions, including egg activation, origination of the zygote centrosome and delivery of paternal factors. Despite this, existing knowledge of the molecular basis of sperm form and function is limited. We used whole-sperm mass spectrometry to identify 381 proteins of the Drosophila melanogaster sperm proteome (DmSP). This approach identified mitochondrial, metabolic and cytoskeletal proteins, in addition to several new functional categories. We also observed nonrandom genomic clustering of sperm genes and underrepresentation on the X chromosome. Identification of widespread functional constraint on the proteome indicates that sexual selection has had a limited role in the overall evolution of D. melanogaster sperm. The relevance of the DmSP to the study of mammalian sperm function and fertilization mechanisms is demonstrated by the identification of substantial homology between the DmSP and proteins of the mouse axoneme accessory structure.

AB - In addition to delivering a haploid genome to the egg, sperm have additional critical functions, including egg activation, origination of the zygote centrosome and delivery of paternal factors. Despite this, existing knowledge of the molecular basis of sperm form and function is limited. We used whole-sperm mass spectrometry to identify 381 proteins of the Drosophila melanogaster sperm proteome (DmSP). This approach identified mitochondrial, metabolic and cytoskeletal proteins, in addition to several new functional categories. We also observed nonrandom genomic clustering of sperm genes and underrepresentation on the X chromosome. Identification of widespread functional constraint on the proteome indicates that sexual selection has had a limited role in the overall evolution of D. melanogaster sperm. The relevance of the DmSP to the study of mammalian sperm function and fertilization mechanisms is demonstrated by the identification of substantial homology between the DmSP and proteins of the mouse axoneme accessory structure.

UR - http://www.scopus.com/inward/record.url?scp=33751510977&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33751510977&partnerID=8YFLogxK

U2 - 10.1038/ng1915

DO - 10.1038/ng1915

M3 - Article

C2 - 17099714

AN - SCOPUS:33751510977

VL - 38

SP - 1440

EP - 1445

JO - Nature Genetics

JF - Nature Genetics

SN - 1061-4036

IS - 12

ER -