Genome-wide study of Pseudomonas aeruginosa outer membrane protein immunogenicity using self-assembling protein microarrays

Wagner R. Montor, Jin Huang, Yanhui Hu, Eugenie Hainsworth, Susan Lynch, Jeannine Weiner Kronish, Claudia L. Ordonez, Tanya Logvinenko, Stephen Lory, Joshua LaBaer

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Pseudomonas aeruginosa is responsible for potentially life-threatening infections in individuals with compromised defense mechanisms and those with cystic fibrosis. P. aeruginosa infection is notable for the appearance of a humoral response to some known antigens, such as flagellin C, elastase, alkaline protease, and others. Although a number of immunogenic proteins are known, no effective vaccine has been approved yet. Here, we report a comprehensive study of all 262 outer membrane and exported P. aeruginosa PAO1 proteins by a modified protein microarray methodology called the nucleic acid-programmable protein array. From this study, it was possible to identify 12 proteins that trigger an adaptive immune response in cystic fibrosis and acutely infected patients, providing valuable information about which bacterial proteins are actually recognized by the immune system in vivo during the natural course of infection. The differential detections of these proteins in patients and controls proved to be statistically significant (P < 0.01). The study provides a list of potential candidates for the improvement of serological diagnostics and the development of vaccines.

Original languageEnglish (US)
Pages (from-to)4877-4886
Number of pages10
JournalInfection and Immunity
Volume77
Issue number11
DOIs
StatePublished - Nov 2009
Externally publishedYes

Fingerprint

Protein Array Analysis
Pseudomonas aeruginosa
Membrane Proteins
Genome
Cystic Fibrosis
Proteins
Vaccines
Flagellin
Pseudomonas Infections
Bacterial Proteins
Pancreatic Elastase
Adaptive Immunity
Infection
Nucleic Acids
Immune System
Antigens
Membranes

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Genome-wide study of Pseudomonas aeruginosa outer membrane protein immunogenicity using self-assembling protein microarrays. / Montor, Wagner R.; Huang, Jin; Hu, Yanhui; Hainsworth, Eugenie; Lynch, Susan; Kronish, Jeannine Weiner; Ordonez, Claudia L.; Logvinenko, Tanya; Lory, Stephen; LaBaer, Joshua.

In: Infection and Immunity, Vol. 77, No. 11, 11.2009, p. 4877-4886.

Research output: Contribution to journalArticle

Montor, WR, Huang, J, Hu, Y, Hainsworth, E, Lynch, S, Kronish, JW, Ordonez, CL, Logvinenko, T, Lory, S & LaBaer, J 2009, 'Genome-wide study of Pseudomonas aeruginosa outer membrane protein immunogenicity using self-assembling protein microarrays', Infection and Immunity, vol. 77, no. 11, pp. 4877-4886. https://doi.org/10.1128/IAI.00698-09
Montor, Wagner R. ; Huang, Jin ; Hu, Yanhui ; Hainsworth, Eugenie ; Lynch, Susan ; Kronish, Jeannine Weiner ; Ordonez, Claudia L. ; Logvinenko, Tanya ; Lory, Stephen ; LaBaer, Joshua. / Genome-wide study of Pseudomonas aeruginosa outer membrane protein immunogenicity using self-assembling protein microarrays. In: Infection and Immunity. 2009 ; Vol. 77, No. 11. pp. 4877-4886.
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