Genome-wide profiling of human cap-independent translation-enhancing elements

Brian P. Wellensiek, Andrew C. Larsen, Bret Stephens, Kim Kukurba, Karl Waern, Natalia Briones, Li Liu, Michael Snyder, Bertram L. Jacobs, Sudhir Kumar, John C. Chaput

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent initiation of translation. This method entails mRNA display of trillions of genomic fragments, selection for initiation of translation and high-throughput deep sequencing. We identified >12,000 translation-enhancing elements (TEEs) in the human genome, generated a high-resolution map of human TEE-bearing regions (TBRs), and validated the function of a subset of sequences in vitro and in cultured cells.

Original languageEnglish (US)
Pages (from-to)747-750
Number of pages4
JournalNature Methods
Volume10
Issue number8
DOIs
StatePublished - Aug 1 2013

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ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Wellensiek, B. P., Larsen, A. C., Stephens, B., Kukurba, K., Waern, K., Briones, N., Liu, L., Snyder, M., Jacobs, B. L., Kumar, S., & Chaput, J. C. (2013). Genome-wide profiling of human cap-independent translation-enhancing elements. Nature Methods, 10(8), 747-750. https://doi.org/10.1038/nmeth.2522