@article{896e7b91dede4ac8ada5d7f20c93cd80,
title = "Genome-wide profiling of human cap-independent translation-enhancing elements",
abstract = "We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent initiation of translation. This method entails mRNA display of trillions of genomic fragments, selection for initiation of translation and high-throughput deep sequencing. We identified >12,000 translation-enhancing elements (TEEs) in the human genome, generated a high-resolution map of human TEE-bearing regions (TBRs), and validated the function of a subset of sequences in vitro and in cultured cells.",
author = "Wellensiek, {Brian P.} and Larsen, {Andrew C.} and Bret Stephens and Kim Kukurba and Karl Waern and Natalia Briones and Li Liu and Michael Snyder and Jacobs, {Bertram L.} and Sudhir Kumar and Chaput, {John C.}",
note = "Funding Information: We thank J. Szostak (Harvard Medical School) and J. Doudna (University of California–Berkeley) for providing the human genome library and luciferase vectors, respectively; N. Pakulis, M. Leon, J. Flores and K. Fenton for technical assistance; G. McInnes for initial bioinformatics analysis; and members of the Chaput laboratory for helpful discussions and comments on the manuscript. This work was supported by US National Institutes of Health to J.C.C. (Eureka Award; GM085530) and S.K. (HG002096-11).",
year = "2013",
month = aug,
doi = "10.1038/nmeth.2522",
language = "English (US)",
volume = "10",
pages = "747--750",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "8",
}