5 Citations (Scopus)

Abstract

Obesity, a state of chronic low-grade inflammation, is strongly associated with the development of hypertension and diabetes. Superoxide, a free radical elevated in obese individuals, promotes hypertension through scavenging the endogenous vasodilator nitric oxide. The hypothesis was a genistein-enriched diet would promote weight loss and reduce oxidative stress and inflammation in the vasculature of intact female ob/ob mice. Aortas and mesenteric arteries were isolated from female ob/ob mice fed genistein-free (0 mg genistein/kg diet; n = 6), standard chow (200–300 mg genistein/kg diet; n = 11) or genistein-enriched (600 mg genistein/kg diet; n = 9) diets for 4 weeks. Sections of isolated vessels were labeled with the superoxide indicator dihydroethidium and fluorescence was measured by confocal microscopy. Protein expression of the inflammatory marker inducible nitric oxide synthase (iNOS) was measured in the perivascular adipose tissue (PVAT) surrounding each vessel and plasma concentrations of superoxide dismutase (SOD) were quantified. Genistein-enriched diet promoted less weight gain compared to animals fed standard chow (P = .008). Standard chow promoted increased superoxide in the aorta (P = .030) and mesenteric arteries (P = .024) compared to a diet devoid of genistein. At all tested concentrations, genistein significantly increased iNOS expression in mesenteric artery PVAT (vs. standard chow, P 

Original languageEnglish (US)
Pages (from-to)789-797
Number of pages9
JournalNutrition Research
Volume36
Issue number8
DOIs
StatePublished - Aug 1 2016

Fingerprint

Genistein
Weight Gain
Oxidative Stress
Inflammation
Diet
Mesenteric Arteries
Superoxides
Nitric Oxide Synthase Type II
Aorta
Adipose Tissue
Hypertension
Vasodilator Agents
Confocal Microscopy
Superoxide Dismutase
Free Radicals
Weight Loss
Nitric Oxide
Obesity
Fluorescence

Keywords

  • Arteries
  • Genistein
  • Obese mouse
  • Obesity
  • Oxidative stress
  • Reactive oxygen species
  • Superoxide

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Genistein supplementation prevents weight gain but promotes oxidative stress and inflammation in the vasculature of female obese ob/ob mice. / Simperova, Anna; Al-Nakkash, Layla; Faust, James J.; Sweazea, Karen.

In: Nutrition Research, Vol. 36, No. 8, 01.08.2016, p. 789-797.

Research output: Contribution to journalArticle

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abstract = "Obesity, a state of chronic low-grade inflammation, is strongly associated with the development of hypertension and diabetes. Superoxide, a free radical elevated in obese individuals, promotes hypertension through scavenging the endogenous vasodilator nitric oxide. The hypothesis was a genistein-enriched diet would promote weight loss and reduce oxidative stress and inflammation in the vasculature of intact female ob/ob mice. Aortas and mesenteric arteries were isolated from female ob/ob mice fed genistein-free (0 mg genistein/kg diet; n = 6), standard chow (200–300 mg genistein/kg diet; n = 11) or genistein-enriched (600 mg genistein/kg diet; n = 9) diets for 4 weeks. Sections of isolated vessels were labeled with the superoxide indicator dihydroethidium and fluorescence was measured by confocal microscopy. Protein expression of the inflammatory marker inducible nitric oxide synthase (iNOS) was measured in the perivascular adipose tissue (PVAT) surrounding each vessel and plasma concentrations of superoxide dismutase (SOD) were quantified. Genistein-enriched diet promoted less weight gain compared to animals fed standard chow (P = .008). Standard chow promoted increased superoxide in the aorta (P = .030) and mesenteric arteries (P = .024) compared to a diet devoid of genistein. At all tested concentrations, genistein significantly increased iNOS expression in mesenteric artery PVAT (vs. standard chow, P ",
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AU - Al-Nakkash, Layla

AU - Faust, James J.

AU - Sweazea, Karen

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N2 - Obesity, a state of chronic low-grade inflammation, is strongly associated with the development of hypertension and diabetes. Superoxide, a free radical elevated in obese individuals, promotes hypertension through scavenging the endogenous vasodilator nitric oxide. The hypothesis was a genistein-enriched diet would promote weight loss and reduce oxidative stress and inflammation in the vasculature of intact female ob/ob mice. Aortas and mesenteric arteries were isolated from female ob/ob mice fed genistein-free (0 mg genistein/kg diet; n = 6), standard chow (200–300 mg genistein/kg diet; n = 11) or genistein-enriched (600 mg genistein/kg diet; n = 9) diets for 4 weeks. Sections of isolated vessels were labeled with the superoxide indicator dihydroethidium and fluorescence was measured by confocal microscopy. Protein expression of the inflammatory marker inducible nitric oxide synthase (iNOS) was measured in the perivascular adipose tissue (PVAT) surrounding each vessel and plasma concentrations of superoxide dismutase (SOD) were quantified. Genistein-enriched diet promoted less weight gain compared to animals fed standard chow (P = .008). Standard chow promoted increased superoxide in the aorta (P = .030) and mesenteric arteries (P = .024) compared to a diet devoid of genistein. At all tested concentrations, genistein significantly increased iNOS expression in mesenteric artery PVAT (vs. standard chow, P 

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