Genetic identification of a putative vinyl chloride reductase in Dehalococcoides sp. strain BAV1

Rosa Krajmalnik-Brown, Tina Hölscher, Ivy N. Thomson, F. Michael Saunders, Kirsti M. Ritalahti, Frank E. Löffler

Research output: Contribution to journalArticle

183 Scopus citations

Abstract

Dehalococcoides sp. strain BAV1 couples growth with the reductive dechlorination of vinyl chloride (VC) to ethene. Degenerate primers targeting conserved regions in reductive dehalogenase (RDase) genes were designed and used to PCR amplify putative RDase genes from strain BAV1. Seven unique RDase gene fragments were identified. Transcription analysis of VC-grown BAV1 cultures suggested that bvcA was involved in VC reductive dechlorination, and the complete sequence of bvcA was obtained. bvcA was absent in Dehalococcoides isolates that failed to respire VC, yet was detected in four of eight VC-respiring mixed cultures.

Original languageEnglish (US)
Pages (from-to)6347-6351
Number of pages5
JournalApplied and environmental microbiology
Volume70
Issue number10
DOIs
StatePublished - Oct 2004

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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