TY - JOUR
T1 - Genetic identification of a putative vinyl chloride reductase in Dehalococcoides sp. strain BAV1
AU - Krajmalnik-Brown, Rosa
AU - Hölscher, Tina
AU - Thomson, Ivy N.
AU - Saunders, F. Michael
AU - Ritalahti, Kirsti M.
AU - Löffler, Frank E.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2004/10
Y1 - 2004/10
N2 - Dehalococcoides sp. strain BAV1 couples growth with the reductive dechlorination of vinyl chloride (VC) to ethene. Degenerate primers targeting conserved regions in reductive dehalogenase (RDase) genes were designed and used to PCR amplify putative RDase genes from strain BAV1. Seven unique RDase gene fragments were identified. Transcription analysis of VC-grown BAV1 cultures suggested that bvcA was involved in VC reductive dechlorination, and the complete sequence of bvcA was obtained. bvcA was absent in Dehalococcoides isolates that failed to respire VC, yet was detected in four of eight VC-respiring mixed cultures.
AB - Dehalococcoides sp. strain BAV1 couples growth with the reductive dechlorination of vinyl chloride (VC) to ethene. Degenerate primers targeting conserved regions in reductive dehalogenase (RDase) genes were designed and used to PCR amplify putative RDase genes from strain BAV1. Seven unique RDase gene fragments were identified. Transcription analysis of VC-grown BAV1 cultures suggested that bvcA was involved in VC reductive dechlorination, and the complete sequence of bvcA was obtained. bvcA was absent in Dehalococcoides isolates that failed to respire VC, yet was detected in four of eight VC-respiring mixed cultures.
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U2 - 10.1128/AEM.70.10.6347-6351.2004
DO - 10.1128/AEM.70.10.6347-6351.2004
M3 - Article
C2 - 15466590
AN - SCOPUS:8144229485
VL - 70
SP - 6347
EP - 6351
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 10
ER -