Genetic engineering of thylakoid protein complexes by chloroplast transformation in Chlamydomonas reinhardtii

Andrew Webber, Scott E. Bingham, Hyeonmoo Lee

Research output: Contribution to journalReview articlepeer-review

10 Scopus citations

Abstract

Chloroplast transformation of Chlamydomonas reinhardtii has developed into a powerful tool for studying the structure, function and assembly of thylakoid protein complexes in a eukaryotic organism. In this article we review the progress that is being made in the development of procedures for efficient chloroplast transformation. This focuses on the development of selectable markers and the use of Chlamydomonas mutants, individually lacking thylakoid protein complexes, as recipients. Chloroplast transformation has now been used to engineer all four major thylakoid protein complexes, photosystem II, photosystem I, cytochrome b6/f and ATP synthase. These results are discussed with an emphasis on new insights into assembly and function of these complexes in chloroplasts as compared with their prokaryotic counterparts.

Original languageEnglish (US)
Pages (from-to)191-205
Number of pages15
JournalPhotosynthesis research
Volume44
Issue number1-2
DOIs
StatePublished - May 1 1995

Keywords

  • chloroplast DNA
  • photosynthesis
  • specific mutagenesis

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

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