Genetic engineering of human pluripotent cells using TALE nucleases

Dirk Hockemeyer, Haoyi Wang, Samira Kiani, Christine S. Lai, Qing Gao, John P. Cassady, Gregory J. Cost, Lei Zhang, Yolanda Santiago, Jeffrey C. Miller, Bryan Zeitler, Jennifer M. Cherone, Xiangdong Meng, Sarah J. Hinkley, Edward J. Rebar, Philip D. Gregory, Fyodor D. Urnov, Rudolf Jaenisch

Research output: Contribution to journalArticle

845 Scopus citations

Abstract

Targeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator-like effector nucleases (TALENs) for five distinct genomic loci. At all loci tested we obtained human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) clones carrying transgenic cassettes solely at the TALEN-specified location. Our data suggest that TALENs employing the specific architectures described here mediate site-specific genome modification in human pluripotent cells with similar efficiency and precision as do zinc-finger nucleases (ZFNs).

Original languageEnglish (US)
Pages (from-to)731-734
Number of pages4
JournalNature biotechnology
Volume29
Issue number8
DOIs
StatePublished - Aug 2011

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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  • Cite this

    Hockemeyer, D., Wang, H., Kiani, S., Lai, C. S., Gao, Q., Cassady, J. P., Cost, G. J., Zhang, L., Santiago, Y., Miller, J. C., Zeitler, B., Cherone, J. M., Meng, X., Hinkley, S. J., Rebar, E. J., Gregory, P. D., Urnov, F. D., & Jaenisch, R. (2011). Genetic engineering of human pluripotent cells using TALE nucleases. Nature biotechnology, 29(8), 731-734. https://doi.org/10.1038/nbt.1927