Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species

Lihua Xiao, Una M. Morgan, Josef Limor, Ananias Escalante, Michael Arrowood, William Shulaw, R. C A Thompson, Ronald Fayer, Altaf A. Lal

Research output: Contribution to journalArticle

418 Citations (Scopus)

Abstract

To assess the genetic diversity in Cryptosporidium parvum, we have sequenced the small subunit (SSU) rRNA gene of seven Cryptosporidium spp., various isolates of C. parvum from eight hosts, and a Cryptosporidium isolate from a desert monitor. Phylogenetic analysis of the SSU rRNA sequences confirmed the multispecies nature of the genus Cryptosporidium, with at least four distinct species (C. parvum, C. baileyi, C. muris, and C. serpentis). Other species previously defined by biologic characteristics, including C. wrairi, C. meleagridis, and C. felis, and the desert monitor isolate, clustered together or within C. parvum. Extensive genetic diversities were present among C. parvum isolates from humans, calves, pigs, dogs, mice, ferrets, marsupials, and a monkey. In general, specific genotypes were associated with specific host species. A PCR-restriction fragment length polymorphism technique previously developed by us could differentiate most Cryptosporidium spp. and C. parvum genotypes, but sequence analysis of the PCR product was needed to differentiate C. wrairi and C. meleagridis from some of the C. parvum genotypes. These results indicate a need for revision in the taxonomy and assessment of the zoonotic potential of some animal C. parvum isolates.

Original languageEnglish (US)
Pages (from-to)3386-3391
Number of pages6
JournalApplied and Environmental Microbiology
Volume65
Issue number8
StatePublished - Aug 1999
Externally publishedYes

Fingerprint

Cryptosporidium parvum
Cryptosporidium
genotype
genetic variation
desert
marsupial
pig
Genotype
polymorphism
phylogenetics
Cryptosporidium baileyi
Cryptosporidium muris
gene
animal
Felis
ribosomal RNA
Marsupialia
Polymerase Chain Reaction
Ferrets
genetic diversity

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Xiao, L., Morgan, U. M., Limor, J., Escalante, A., Arrowood, M., Shulaw, W., ... Lal, A. A. (1999). Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species. Applied and Environmental Microbiology, 65(8), 3386-3391.

Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species. / Xiao, Lihua; Morgan, Una M.; Limor, Josef; Escalante, Ananias; Arrowood, Michael; Shulaw, William; Thompson, R. C A; Fayer, Ronald; Lal, Altaf A.

In: Applied and Environmental Microbiology, Vol. 65, No. 8, 08.1999, p. 3386-3391.

Research output: Contribution to journalArticle

Xiao, L, Morgan, UM, Limor, J, Escalante, A, Arrowood, M, Shulaw, W, Thompson, RCA, Fayer, R & Lal, AA 1999, 'Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species', Applied and Environmental Microbiology, vol. 65, no. 8, pp. 3386-3391.
Xiao L, Morgan UM, Limor J, Escalante A, Arrowood M, Shulaw W et al. Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species. Applied and Environmental Microbiology. 1999 Aug;65(8):3386-3391.
Xiao, Lihua ; Morgan, Una M. ; Limor, Josef ; Escalante, Ananias ; Arrowood, Michael ; Shulaw, William ; Thompson, R. C A ; Fayer, Ronald ; Lal, Altaf A. / Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species. In: Applied and Environmental Microbiology. 1999 ; Vol. 65, No. 8. pp. 3386-3391.
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