Genetic diversity and host range studies of turnip curly top virus

Sara Razavinejad, Jahangir Heydarnejad, Mehdi Kamali, Hossain Massumi, Simona Kraberger, Arvind Varsani

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Turnip curly top virus (TCTV) is a unique geminivirus that has recently been characterised as infecting turnips in Iran. The genome of TCTV shares <68 % pairwise identity with other geminiviruses and has a genome organisation similar to that of curtoviruses and topocuvirus. The replication-associated protein (Rep) bears the highest similarity to curtovirus Reps (48.5-69.0 %); however, in the case of the capsid protein (CP), the extent of similarity is only 39.5-44.5 %. We constructed an agroinfectious clone of TCTV and undertook host range studies on ten plant species; in three species (turnip, sugar beet and cowpea), we detected infection which presents curly top symptoms in turnip and sugar beet. The efficiency of TCTV infection in agroinoculated turnip plants was 71.7 %, and the infection was successfully transmitted to 80 % of the healthy turnip plants used in the insect transmission studies by Circulifer haematoceps under greenhouse conditions. We also determined the genome sequence of 14 new TCTV isolates from southern Iran isolated from turnips. We observed ~13 % diversity amongst all the TCTV isolates and found evidence of recombination in the CP- and Rep-coding regions of the genomes.

Original languageEnglish (US)
Pages (from-to)345-353
Number of pages9
JournalVirus Genes
Volume46
Issue number2
DOIs
StatePublished - Apr 1 2013
Externally publishedYes

Keywords

  • Curly top disease
  • Geminivirus
  • Iran
  • Turnip curly top virus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

Fingerprint Dive into the research topics of 'Genetic diversity and host range studies of turnip curly top virus'. Together they form a unique fingerprint.

  • Cite this

    Razavinejad, S., Heydarnejad, J., Kamali, M., Massumi, H., Kraberger, S., & Varsani, A. (2013). Genetic diversity and host range studies of turnip curly top virus. Virus Genes, 46(2), 345-353. https://doi.org/10.1007/s11262-012-0858-y