Genetic diversity and distribution of tomato-infecting begomoviruses in Iran

Roya Fazeli, Jahangir Heydarnejad, Hossain Massumi, Mahdi Shaabanian, Arvind Varsani

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The incidence and severity of tomato leaf curl disease (TLCD) is increasing worldwide. Here we assess the diversity and distribution within tomato producing areas of Iran of begomoviruses that cause this disease. Tomato with typical TLCD symptoms and asymptomatic weeds were collected in 2005 and 2006 and tested for the presence of begomovirus DNA using polymerase chain reaction (PCR). Analysis of cloned and sequenced PCR products revealed that both mono- and bipartite begomoviruses are associated with TLCD in Iran. Furthermore, our results confirmed the symptomless infection with mono- and bipartite begomoviruses of two weed species, Chrozophora hierosolymitana Spreng (Euphobiaceae) and Herniaria sp. (Caryophyllaceae). Eighteen Iranian begomovirus isolates were classified into two major groups and two or three subgroups according to the 5′-proximal 200 nucleotides of the coat protein (CP) gene or the N-terminal 600 nucleotides of the Rep gene. Whereas most of the monopartite isolates showed closest similarity to tomato yellow leaf curl virus-Gezira (TYLCV-Ge), the three bipartite isolates were most similar to Tomato leaf curl New Delhi virus (ToLCNDV). Mixed mono- and a bipartite begomovirus infections were detected in both tomato and C. hierosolymitana. Our results indicate that the tomato producing areas in central, southern, and southeastern Iran are threatened by begomoviruses originating from both the Mediterranean basin and the Indian subcontinent.

Original languageEnglish (US)
Pages (from-to)311-319
Number of pages9
JournalVirus Genes
Volume38
Issue number2
DOIs
StatePublished - Apr 2009
Externally publishedYes

Fingerprint

Begomovirus
Lycopersicon esculentum
Iran
Caryophyllaceae
Nucleotides
Viruses
Asymptomatic Diseases
Polymerase Chain Reaction
Capsid Proteins
DNA-Directed DNA Polymerase
Infection
Genes

Keywords

  • Begomovirus
  • Geminivirus
  • Mixed infection
  • TLCV
  • Tomato leaf curl disease

ASJC Scopus subject areas

  • Virology
  • Genetics
  • Molecular Biology

Cite this

Genetic diversity and distribution of tomato-infecting begomoviruses in Iran. / Fazeli, Roya; Heydarnejad, Jahangir; Massumi, Hossain; Shaabanian, Mahdi; Varsani, Arvind.

In: Virus Genes, Vol. 38, No. 2, 04.2009, p. 311-319.

Research output: Contribution to journalArticle

Fazeli, R, Heydarnejad, J, Massumi, H, Shaabanian, M & Varsani, A 2009, 'Genetic diversity and distribution of tomato-infecting begomoviruses in Iran', Virus Genes, vol. 38, no. 2, pp. 311-319. https://doi.org/10.1007/s11262-008-0310-5
Fazeli, Roya ; Heydarnejad, Jahangir ; Massumi, Hossain ; Shaabanian, Mahdi ; Varsani, Arvind. / Genetic diversity and distribution of tomato-infecting begomoviruses in Iran. In: Virus Genes. 2009 ; Vol. 38, No. 2. pp. 311-319.
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