Genetic distance in housekeeping genes between Plasmodium falciparum and Plasmodium reichenowi and Within P. falciparum

Kazuyuki Tanabe, Naoko Sakihama, Tetsuya Hattori, Lisa Ranford-Cartwright, Ira Goldman, Ananias A. Escalante, Altaf A. Lal

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The time to the most recent common ancestor of the extant populations of Plasmodium falciparum is controversial. The controversy primarily stems from the limited availability of sequences from Plasmodium reichenowi, a chimpanzee malaria parasite closely related to P. falciparum. Since the rate of nucleotide substitution differs in different loci and DNA regions, the estimation of genetic distance between P. falciparum and P. reichenowi should be performed using orthologous sequences that are evolving neutrally. Here, we obtained full-length sequences of two housekeeping genes, sarcoplasmic and endoplasmic reticulum Ca2+-ATPase (serca) and lactate dehydrogenase (ldh), from 11 isolates of P. falciparum and 1 isolate of P. reichenowi and estimate the interspecific genetic distance (divergence) between the two species and intraspecific genetic distance (polymorphism) within P. falciparum. Interspecific distance and intraspecific distance at synonymous sites of interspecies-conserved regions of serca and ldh were 0.0672 ± 0.0088 and 0.0011 ± 0.0007, respectively, using the Nei and Gojobori method. Based on the ratio of interspecific distance to intraspecific distance, the time to the most recent common ancestor of P. falciparum was estimated to be (8.30 ± 5.40) × 104 and (11.62 ± 7.56) × 10 4 years ago, assuming the divergence time of the two parasite species to be 5 and 7 million years ago, respectively.

Original languageEnglish (US)
Pages (from-to)687-694
Number of pages8
JournalJournal of Molecular Evolution
Volume59
Issue number5
DOIs
StatePublished - Nov 2004
Externally publishedYes

Fingerprint

Plasmodium
Calcium-Transporting ATPases
Essential Genes
Plasmodium falciparum
L-Lactate Dehydrogenase
genetic distance
Genes
common ancestry
gene
parasite
Polymorphism
divergence
sarcoplasmic reticulum
Substitution reactions
Ca2-transporting ATPase
Nucleotides
malaria
Availability
Sarcoplasmic Reticulum
lactate dehydrogenase

Keywords

  • Genetic distance
  • Lactate dehydrogenase
  • Malaria parasite
  • Most recent common ancestor
  • Plasmodium falciparum
  • Plasmodium reichenowi
  • Polymorphism
  • Sarcoplasmic and endoplasmic reticulum Ca ATPase

ASJC Scopus subject areas

  • Genetics
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Genetics(clinical)
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Tanabe, K., Sakihama, N., Hattori, T., Ranford-Cartwright, L., Goldman, I., Escalante, A. A., & Lal, A. A. (2004). Genetic distance in housekeeping genes between Plasmodium falciparum and Plasmodium reichenowi and Within P. falciparum. Journal of Molecular Evolution, 59(5), 687-694. https://doi.org/10.1007/s00239-004-2662-3

Genetic distance in housekeeping genes between Plasmodium falciparum and Plasmodium reichenowi and Within P. falciparum. / Tanabe, Kazuyuki; Sakihama, Naoko; Hattori, Tetsuya; Ranford-Cartwright, Lisa; Goldman, Ira; Escalante, Ananias A.; Lal, Altaf A.

In: Journal of Molecular Evolution, Vol. 59, No. 5, 11.2004, p. 687-694.

Research output: Contribution to journalArticle

Tanabe, K, Sakihama, N, Hattori, T, Ranford-Cartwright, L, Goldman, I, Escalante, AA & Lal, AA 2004, 'Genetic distance in housekeeping genes between Plasmodium falciparum and Plasmodium reichenowi and Within P. falciparum', Journal of Molecular Evolution, vol. 59, no. 5, pp. 687-694. https://doi.org/10.1007/s00239-004-2662-3
Tanabe, Kazuyuki ; Sakihama, Naoko ; Hattori, Tetsuya ; Ranford-Cartwright, Lisa ; Goldman, Ira ; Escalante, Ananias A. ; Lal, Altaf A. / Genetic distance in housekeeping genes between Plasmodium falciparum and Plasmodium reichenowi and Within P. falciparum. In: Journal of Molecular Evolution. 2004 ; Vol. 59, No. 5. pp. 687-694.
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