Genetic characterization of specific pathogen-free rhesus macaque (Macaca Mulatta) populations at the California National Primate Research Center (CNPRC)

Sree Kanthaswamy, Alex Kou, Jessica Satkoski, Maria Cecilia T. Penedo, Ward Thea, Jillian Ng, Leanne Gill, Nicholas W. Lerche, Bethany J.A. Erickson, Glenn Smith David

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25 Scopus citations

Abstract

A study based on 14 STRs was conducted to understand intergenerational genetic changes that have occurred within the California National Primate Research Center's (CNPRC) regular specific pathogenfree (SPF) and super-SPF captive rhesus macaque populations relative to their conventional founders. Intergenerational genetic drift has caused age cohorts of each study population, especially within the conventional population, to become increasingly differentiated from each other and from their founders. Although there is still only minimal stratification between the conventional population and either of the two SPF populations, separate derivation of the regular and super-SPF animals from their conventional founders has caused the two SPF populations to remain marginally different from each other. The regular SPF and, especially, the super-SPF populations have been influenced by the effects of differential ancestry, sampling, and lost rare alleles, causing a substantial degree of genetic divergence between these subpopulations. The country of origin of founders is the principal determinant of the MHC haplotype composition of the SPF stocks at the CNPRC. Selection of SPF colony breeders bearing desired genotypes of Mamu-A*01 or -B*01 has not affected the overall genetic heterogeneity of the conventional and the SPF research stocks. Because misclassifying the ancestry of research stocks can undermine experimental outcomes by excluding animals with regional-specific genotypes or phenotypes of importance, understanding founder/descendent genetic relationships is crucial for investigating candidate genes with distinct geographic origins. Together with demographic management, population genetic assessments of SPF colonies can curtail excessive phenotypic variation among the study stocks and facilitate successful production goals.

Original languageEnglish (US)
Pages (from-to)587-599
Number of pages13
JournalAmerican Journal of Primatology
Volume72
Issue number7
DOIs
StatePublished - Jun 1 2010

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Keywords

  • Colony management
  • Genetic management
  • Genetic structure
  • Major histocompatibility complex (MHC)
  • Microsatellites

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Animal Science and Zoology

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