Generation of transgenic banana (Musa acuminata) plants via Agrobacterium-mediated transformation

Gregory D. May, Rownak Afza, Hugh Mason, Alicja Wiecko, Frantisek J. Novak, Charles J. Arntzen

Research output: Contribution to journalArticle

192 Citations (Scopus)

Abstract

An Agrobacterium-mediated plant transformation system was developed for the generation of transgenic banana (Musa spp. van Grand Nain). This system allowed for the recovery of putative transformants within four weeks after co-cultivation of tissue samples with Agrobacterium. Two or more cycles of meristem rooting and micropropagation allowed for the selection of plants from this putative transformant population which demonstrated chromosomal integration of foreign DNA by Southern analysis with no indication of chimeric tissues. Since plant breeding strategies aimed at banana crop improvement are extremely complex and long-term, virtually all commercial production is from clonal derivatives of naturally occurring variants. The genetic transformation technology reported herein will provide an additional tool for crop breeders who wish to introduce value-added traits into the banana and plantain cultivars that serve as vital food sources and a means of generating export income for producing nations.

Original languageEnglish (US)
Pages (from-to)486-492
Number of pages7
JournalNature Biotechnology
Volume13
Issue number5
StatePublished - 1995
Externally publishedYes

Fingerprint

Agrobacterium
Musa
Crops
Tissue
DNA
Plantago
Derivatives
Genetic Transformation
Recovery
Meristem
Technology
Food
Population

ASJC Scopus subject areas

  • Microbiology
  • Biotechnology

Cite this

May, G. D., Afza, R., Mason, H., Wiecko, A., Novak, F. J., & Arntzen, C. J. (1995). Generation of transgenic banana (Musa acuminata) plants via Agrobacterium-mediated transformation. Nature Biotechnology, 13(5), 486-492.

Generation of transgenic banana (Musa acuminata) plants via Agrobacterium-mediated transformation. / May, Gregory D.; Afza, Rownak; Mason, Hugh; Wiecko, Alicja; Novak, Frantisek J.; Arntzen, Charles J.

In: Nature Biotechnology, Vol. 13, No. 5, 1995, p. 486-492.

Research output: Contribution to journalArticle

May, GD, Afza, R, Mason, H, Wiecko, A, Novak, FJ & Arntzen, CJ 1995, 'Generation of transgenic banana (Musa acuminata) plants via Agrobacterium-mediated transformation', Nature Biotechnology, vol. 13, no. 5, pp. 486-492.
May, Gregory D. ; Afza, Rownak ; Mason, Hugh ; Wiecko, Alicja ; Novak, Frantisek J. ; Arntzen, Charles J. / Generation of transgenic banana (Musa acuminata) plants via Agrobacterium-mediated transformation. In: Nature Biotechnology. 1995 ; Vol. 13, No. 5. pp. 486-492.
@article{68fc829dfbfe43629e0a9e6d5caf592a,
title = "Generation of transgenic banana (Musa acuminata) plants via Agrobacterium-mediated transformation",
abstract = "An Agrobacterium-mediated plant transformation system was developed for the generation of transgenic banana (Musa spp. van Grand Nain). This system allowed for the recovery of putative transformants within four weeks after co-cultivation of tissue samples with Agrobacterium. Two or more cycles of meristem rooting and micropropagation allowed for the selection of plants from this putative transformant population which demonstrated chromosomal integration of foreign DNA by Southern analysis with no indication of chimeric tissues. Since plant breeding strategies aimed at banana crop improvement are extremely complex and long-term, virtually all commercial production is from clonal derivatives of naturally occurring variants. The genetic transformation technology reported herein will provide an additional tool for crop breeders who wish to introduce value-added traits into the banana and plantain cultivars that serve as vital food sources and a means of generating export income for producing nations.",
author = "May, {Gregory D.} and Rownak Afza and Hugh Mason and Alicja Wiecko and Novak, {Frantisek J.} and Arntzen, {Charles J.}",
year = "1995",
language = "English (US)",
volume = "13",
pages = "486--492",
journal = "Biotechnology",
issn = "1087-0156",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - Generation of transgenic banana (Musa acuminata) plants via Agrobacterium-mediated transformation

AU - May, Gregory D.

AU - Afza, Rownak

AU - Mason, Hugh

AU - Wiecko, Alicja

AU - Novak, Frantisek J.

AU - Arntzen, Charles J.

PY - 1995

Y1 - 1995

N2 - An Agrobacterium-mediated plant transformation system was developed for the generation of transgenic banana (Musa spp. van Grand Nain). This system allowed for the recovery of putative transformants within four weeks after co-cultivation of tissue samples with Agrobacterium. Two or more cycles of meristem rooting and micropropagation allowed for the selection of plants from this putative transformant population which demonstrated chromosomal integration of foreign DNA by Southern analysis with no indication of chimeric tissues. Since plant breeding strategies aimed at banana crop improvement are extremely complex and long-term, virtually all commercial production is from clonal derivatives of naturally occurring variants. The genetic transformation technology reported herein will provide an additional tool for crop breeders who wish to introduce value-added traits into the banana and plantain cultivars that serve as vital food sources and a means of generating export income for producing nations.

AB - An Agrobacterium-mediated plant transformation system was developed for the generation of transgenic banana (Musa spp. van Grand Nain). This system allowed for the recovery of putative transformants within four weeks after co-cultivation of tissue samples with Agrobacterium. Two or more cycles of meristem rooting and micropropagation allowed for the selection of plants from this putative transformant population which demonstrated chromosomal integration of foreign DNA by Southern analysis with no indication of chimeric tissues. Since plant breeding strategies aimed at banana crop improvement are extremely complex and long-term, virtually all commercial production is from clonal derivatives of naturally occurring variants. The genetic transformation technology reported herein will provide an additional tool for crop breeders who wish to introduce value-added traits into the banana and plantain cultivars that serve as vital food sources and a means of generating export income for producing nations.

UR - http://www.scopus.com/inward/record.url?scp=0029022626&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029022626&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0029022626

VL - 13

SP - 486

EP - 492

JO - Biotechnology

JF - Biotechnology

SN - 1087-0156

IS - 5

ER -