Generation of regionally specific neural progenitor cells (NPCs) and neurons from human pluripotent stem cells (HPSCs)

Josh Cutts, Nicholas Brookhouser, David Brafman

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

Neural progenitor cells (NPCs) derived from human pluripotent stem cells (hPSCs) are a multipotent cell population capable of long-term expansion and differentiation into a variety of neuronal subtypes. As such, NPCs have tremendous potential for disease modeling, drug screening, and regenerative medicine. Current methods for the generation of NPCs results in cell populations homogenous for pan-neural markers such as SOX1 and SOX2 but heterogeneous with respect to regional identity. In order to use NPCs and their neuronal derivatives to investigate mechanisms of neurological disorders and develop more physiologically relevant disease models, methods for generation of regionally specific NPCs and neurons are needed. Here, we describe a protocol in which exogenous manipulation of WNT signaling, through either activation or inhibition, during neural differentiation of hPSCs, promotes the formation of regionally homogenous NPCs and neuronal cultures. In addition, we provide methods to monitor and characterize the efficiency of hPSC differentiation to these regionally specific cell identities.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages121-144
Number of pages24
Volume1516
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1516
ISSN (Print)10643745

Keywords

  • Anterior–posterior patterning
  • Disease modeling
  • Drug screening
  • Human pluripotent stem cells
  • Neural progenitor cells
  • Neurons
  • Regenerative medicine

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Biology
  • Genetics

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