Generation of polypeptide-templated gold nanoparticles using ionizing radiation

Candace Rae Walker, Karthik Pushpavanam, Divya Geetha Nair, Thrimoorthy Potta, Caesario Sutiyoso, Vikram Kodibagkar, Stephen Sapareto, John Chang, Kaushal Rege

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Ionizing radiation, including γ rays and X-rays, are high-energy electromagnetic radiation with diverse applications in nuclear energy, astrophysics, and medicine. In this work, we describe the use of ionizing radiation and cysteine-containing elastin-like polypeptides (CnELPs, where n = 2 or 12 cysteines in the polypeptide sequence) for the generation of gold nanoparticles. In the presence of CnELPs, ionizing radiation doses higher than 175 Gy resulted in the formation of maroon-colored gold nanoparticle dispersions, with maximal absorbance at 520 nm, from colorless metal salts. Visible color changes were not observed in any of the control systems, indicating that ionizing radiation, gold salt solution, and C nELPs were all required for nanoparticle formation. The hydrodynamic diameters of nanoparticles, determined using dynamic light scattering, were in the range of 80-150 nm, while TEM imaging indicated the formation of gold cores 10-20 nm in diameter. Interestingly, C2ELPs formed 1-2 nm diameter gold nanoparticles in the absence of radiation. Our results describe a facile method of nanoparticle formation in which nanoparticle size can be tailored based on radiation dose and CnELP type. Further improvements in these polypeptide-based systems can lead to colorimetric detection of ionizing radiation in a variety of applications.

Original languageEnglish (US)
Pages (from-to)10166-10173
Number of pages8
JournalLangmuir
Volume29
Issue number32
DOIs
StatePublished - Aug 13 2013

Fingerprint

Polypeptides
Ionizing radiation
polypeptides
ionizing radiation
Gold
gold
Nanoparticles
nanoparticles
Peptides
cysteine
Dosimetry
Cysteine
Salts
elastin
salts
Elastin
dosage
Astrophysics
Dynamic light scattering
radiation

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Walker, C. R., Pushpavanam, K., Nair, D. G., Potta, T., Sutiyoso, C., Kodibagkar, V., ... Rege, K. (2013). Generation of polypeptide-templated gold nanoparticles using ionizing radiation. Langmuir, 29(32), 10166-10173. https://doi.org/10.1021/la400567d

Generation of polypeptide-templated gold nanoparticles using ionizing radiation. / Walker, Candace Rae; Pushpavanam, Karthik; Nair, Divya Geetha; Potta, Thrimoorthy; Sutiyoso, Caesario; Kodibagkar, Vikram; Sapareto, Stephen; Chang, John; Rege, Kaushal.

In: Langmuir, Vol. 29, No. 32, 13.08.2013, p. 10166-10173.

Research output: Contribution to journalArticle

Walker, CR, Pushpavanam, K, Nair, DG, Potta, T, Sutiyoso, C, Kodibagkar, V, Sapareto, S, Chang, J & Rege, K 2013, 'Generation of polypeptide-templated gold nanoparticles using ionizing radiation', Langmuir, vol. 29, no. 32, pp. 10166-10173. https://doi.org/10.1021/la400567d
Walker CR, Pushpavanam K, Nair DG, Potta T, Sutiyoso C, Kodibagkar V et al. Generation of polypeptide-templated gold nanoparticles using ionizing radiation. Langmuir. 2013 Aug 13;29(32):10166-10173. https://doi.org/10.1021/la400567d
Walker, Candace Rae ; Pushpavanam, Karthik ; Nair, Divya Geetha ; Potta, Thrimoorthy ; Sutiyoso, Caesario ; Kodibagkar, Vikram ; Sapareto, Stephen ; Chang, John ; Rege, Kaushal. / Generation of polypeptide-templated gold nanoparticles using ionizing radiation. In: Langmuir. 2013 ; Vol. 29, No. 32. pp. 10166-10173.
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