Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells

Nathan Kumar, Jenna Richter, Josh Cutts, Kevin T. Bush, Cleber Trujillo, Sanjay K. Nigam, Terry Gaasterland, David Brafman, Karl Willert

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The field of tissue engineering entered a new era with the development of human pluripotent stem cells (hPSCs), which are capable of unlimited expansion whilst retaining the potential to differentiate into all mature cell populations. However, these cells harbor significant risks, including tumor formation upon transplantation. One way to mitigate this risk is to develop expandable progenitor cell populations with restricted differentiation potential. Here, we used a cellular microarray technology to identify a defined and optimized culture condition that supports the derivation and propagation of a cell population with mesodermal properties. This cell population, referred to as intermediate mesodermal progenitor (IMP) cells, is capable of unlimited expansion, lacks tumor formation potential, and, upon appropriate stimulation, readily acquires properties of a sub-population of kidney cells. Interestingly, IMP cells fail to differentiate into other mesodermally- derived tissues, including blood and heart, suggesting that these cells are restricted to an intermediate mesodermal fate.

Original languageEnglish (US)
Article numbere08413
JournaleLife
Volume4
Issue numberNOVEMBER2015
DOIs
StatePublished - Nov 10 2015

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Pluripotent Stem Cells
Mesoderm
Stem cells
Stem Cells
Cells
Cell Line
Tumors
Population
Microarrays
Ports and harbors
Tissue engineering
Blood
Human Development
Tissue Engineering
Tissue
Neoplasms
Transplantation
Technology
Kidney

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)
  • Neuroscience(all)

Cite this

Kumar, N., Richter, J., Cutts, J., Bush, K. T., Trujillo, C., Nigam, S. K., ... Willert, K. (2015). Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells. eLife, 4(NOVEMBER2015), [e08413]. https://doi.org/10.7554/eLife.08413

Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells. / Kumar, Nathan; Richter, Jenna; Cutts, Josh; Bush, Kevin T.; Trujillo, Cleber; Nigam, Sanjay K.; Gaasterland, Terry; Brafman, David; Willert, Karl.

In: eLife, Vol. 4, No. NOVEMBER2015, e08413, 10.11.2015.

Research output: Contribution to journalArticle

Kumar, N, Richter, J, Cutts, J, Bush, KT, Trujillo, C, Nigam, SK, Gaasterland, T, Brafman, D & Willert, K 2015, 'Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells', eLife, vol. 4, no. NOVEMBER2015, e08413. https://doi.org/10.7554/eLife.08413
Kumar N, Richter J, Cutts J, Bush KT, Trujillo C, Nigam SK et al. Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells. eLife. 2015 Nov 10;4(NOVEMBER2015). e08413. https://doi.org/10.7554/eLife.08413
Kumar, Nathan ; Richter, Jenna ; Cutts, Josh ; Bush, Kevin T. ; Trujillo, Cleber ; Nigam, Sanjay K. ; Gaasterland, Terry ; Brafman, David ; Willert, Karl. / Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells. In: eLife. 2015 ; Vol. 4, No. NOVEMBER2015.
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