Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells

Nathan Kumar, Jenna Richter, Josh Cutts, Kevin T. Bush, Cleber Trujillo, Sanjay K. Nigam, Terry Gaasterland, David Brafman, Karl Willert

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The field of tissue engineering entered a new era with the development of human pluripotent stem cells (hPSCs), which are capable of unlimited expansion whilst retaining the potential to differentiate into all mature cell populations. However, these cells harbor significant risks, including tumor formation upon transplantation. One way to mitigate this risk is to develop expandable progenitor cell populations with restricted differentiation potential. Here, we used a cellular microarray technology to identify a defined and optimized culture condition that supports the derivation and propagation of a cell population with mesodermal properties. This cell population, referred to as intermediate mesodermal progenitor (IMP) cells, is capable of unlimited expansion, lacks tumor formation potential, and, upon appropriate stimulation, readily acquires properties of a sub-population of kidney cells. Interestingly, IMP cells fail to differentiate into other mesodermally- derived tissues, including blood and heart, suggesting that these cells are restricted to an intermediate mesodermal fate.

Original languageEnglish (US)
Article numbere08413
JournaleLife
Volume4
Issue numberNOVEMBER2015
DOIs
StatePublished - Nov 10 2015

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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    Kumar, N., Richter, J., Cutts, J., Bush, K. T., Trujillo, C., Nigam, S. K., Gaasterland, T., Brafman, D., & Willert, K. (2015). Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells. eLife, 4(NOVEMBER2015), [e08413]. https://doi.org/10.7554/eLife.08413