Gene expression responses in male fathead minnows exposed to binary mixtures of an estrogen and antiestrogen

Natàlia Garcia-Reyero, Kevin J. Kroll, Li Liu, Edward F. Orlando, Karen Watanabe-Sailor, María S. Sepúlveda, Daniel L. Villeneuve, Edward J. Perkins, Gerald T. Ankley, Nancy D. Denslow

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Background: Aquatic organisms are continuously exposed to complex mixtures of chemicals, many of which can interfere with their endocrine system, resulting in impaired reproduction, development or survival, among others. In order to analyze the effects and mechanisms of action of estrogen/anti-estrogen mixtures, we exposed male fathead minnows (Pimephales promelas) for 48 hours via the water to 2, 5, 10, and 50 ng 17α-ethinylestradiol (EE2)/L, 100 ng ZM 189,154/L (a potent antiestrogen known to block activity of estrogen receptors) or mixtures of 5 or 50 ng EE2/L with 100 ng ZM 189,154/L. We analyzed gene expression changes in the gonad, as well as hormone and vitellogenin plasma levels. Results: Steroidogenesis was down-regulated by EE2 as reflected by the reduced plasma levels of testosterone in the exposed fish and down-regulation of genes in the steroidogenic pathway. Microarray analysis of testis of fathead minnows treated with 5 ng EE2/L or with the mixture of 5 ng EE2/L and 100 ng ZM 189,154/L indicated that some of the genes whose expression was changed by EE2 were blocked by ZM 189,154, while others were either not blocked or enhanced by the mixture, generating two distinct expression patterns. Gene ontology and pathway analysis programs were used to determine categories of genes for each expression pattern. Conclusion: Our results suggest that response to estrogens occurs via multiple mechanisms, including canonical binding to soluble estrogen receptors, membrane estrogen receptors, and other mechanisms that are not blocked by pure antiestrogens.

Original languageEnglish (US)
Article number308
JournalBMC Genomics
Volume10
DOIs
StatePublished - Jul 13 2009
Externally publishedYes

Fingerprint

Cyprinidae
Estrogen Receptor Modulators
Estrogens
Estrogen Receptors
Gene Expression
Vitellogenins
Aquatic Organisms
Gene Ontology
Ethinyl Estradiol
Endocrine System
Gonads
Microarray Analysis
Complex Mixtures
Reproduction
Testosterone
Testis
Fishes
Down-Regulation
Hormones
Membranes

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

Cite this

Gene expression responses in male fathead minnows exposed to binary mixtures of an estrogen and antiestrogen. / Garcia-Reyero, Natàlia; Kroll, Kevin J.; Liu, Li; Orlando, Edward F.; Watanabe-Sailor, Karen; Sepúlveda, María S.; Villeneuve, Daniel L.; Perkins, Edward J.; Ankley, Gerald T.; Denslow, Nancy D.

In: BMC Genomics, Vol. 10, 308, 13.07.2009.

Research output: Contribution to journalArticle

Garcia-Reyero, N, Kroll, KJ, Liu, L, Orlando, EF, Watanabe-Sailor, K, Sepúlveda, MS, Villeneuve, DL, Perkins, EJ, Ankley, GT & Denslow, ND 2009, 'Gene expression responses in male fathead minnows exposed to binary mixtures of an estrogen and antiestrogen', BMC Genomics, vol. 10, 308. https://doi.org/10.1186/1471-2164-10-308
Garcia-Reyero, Natàlia ; Kroll, Kevin J. ; Liu, Li ; Orlando, Edward F. ; Watanabe-Sailor, Karen ; Sepúlveda, María S. ; Villeneuve, Daniel L. ; Perkins, Edward J. ; Ankley, Gerald T. ; Denslow, Nancy D. / Gene expression responses in male fathead minnows exposed to binary mixtures of an estrogen and antiestrogen. In: BMC Genomics. 2009 ; Vol. 10.
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AU - Watanabe-Sailor, Karen

AU - Sepúlveda, María S.

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