Gender differences in the validity of testosterone measured in saliva by immunoassay

Elizabeth A. Shirtcliff, Douglas A. Granger, Andrea Likos

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

We rigorously evaluated gender differences in the measurement validity of salivary testosterone. Matched serum, saliva, and finger stick blood spot specimens were collected from 40 (20 males) young adults (aged 18-27 years). Saliva was assayed for testosterone by two independent (isotopic and non-isotopic) immunoassay methods. Serum was assayed by commercially available immunoassay kits for free and total testosterone. An immunoassay was developed for the measurement of testosterone in dried blood spots and is presented in detail so as to be reproducible from this report. Regardless of assay method, salivary testosterone levels are modestly correlated with serum levels for males but not necessarily for females. Blood spot assay results were highly correlated with serum total and free testosterone for both males and females. Substitution of saliva assay results for serum values substantially underestimates known testosterone-behavior associations, and this effect is much more pronounced for females than for males. The findings have important implications for the use and potential misuse of noninvasive measures of testosterone, and with respect to statistical power, the probability of observing significant testosterone-behavior relationships.

Original languageEnglish (US)
Pages (from-to)62-69
Number of pages8
JournalHormones and Behavior
Volume42
Issue number1
DOIs
StatePublished - Aug 2002
Externally publishedYes

Fingerprint

Saliva
Immunoassay
Testosterone
Serum
Fingers
Young Adult

ASJC Scopus subject areas

  • Endocrinology
  • Behavioral Neuroscience
  • Neurology
  • Psychology(all)

Cite this

Gender differences in the validity of testosterone measured in saliva by immunoassay. / Shirtcliff, Elizabeth A.; Granger, Douglas A.; Likos, Andrea.

In: Hormones and Behavior, Vol. 42, No. 1, 08.2002, p. 62-69.

Research output: Contribution to journalArticle

Shirtcliff, Elizabeth A. ; Granger, Douglas A. ; Likos, Andrea. / Gender differences in the validity of testosterone measured in saliva by immunoassay. In: Hormones and Behavior. 2002 ; Vol. 42, No. 1. pp. 62-69.
@article{065abe0d61cc425d8459a28d36dff237,
title = "Gender differences in the validity of testosterone measured in saliva by immunoassay",
abstract = "We rigorously evaluated gender differences in the measurement validity of salivary testosterone. Matched serum, saliva, and finger stick blood spot specimens were collected from 40 (20 males) young adults (aged 18-27 years). Saliva was assayed for testosterone by two independent (isotopic and non-isotopic) immunoassay methods. Serum was assayed by commercially available immunoassay kits for free and total testosterone. An immunoassay was developed for the measurement of testosterone in dried blood spots and is presented in detail so as to be reproducible from this report. Regardless of assay method, salivary testosterone levels are modestly correlated with serum levels for males but not necessarily for females. Blood spot assay results were highly correlated with serum total and free testosterone for both males and females. Substitution of saliva assay results for serum values substantially underestimates known testosterone-behavior associations, and this effect is much more pronounced for females than for males. The findings have important implications for the use and potential misuse of noninvasive measures of testosterone, and with respect to statistical power, the probability of observing significant testosterone-behavior relationships.",
author = "Shirtcliff, {Elizabeth A.} and Granger, {Douglas A.} and Andrea Likos",
year = "2002",
month = "8",
doi = "10.1006/hbeh.2002.1798",
language = "English (US)",
volume = "42",
pages = "62--69",
journal = "Hormones and Behavior",
issn = "0018-506X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Gender differences in the validity of testosterone measured in saliva by immunoassay

AU - Shirtcliff, Elizabeth A.

AU - Granger, Douglas A.

AU - Likos, Andrea

PY - 2002/8

Y1 - 2002/8

N2 - We rigorously evaluated gender differences in the measurement validity of salivary testosterone. Matched serum, saliva, and finger stick blood spot specimens were collected from 40 (20 males) young adults (aged 18-27 years). Saliva was assayed for testosterone by two independent (isotopic and non-isotopic) immunoassay methods. Serum was assayed by commercially available immunoassay kits for free and total testosterone. An immunoassay was developed for the measurement of testosterone in dried blood spots and is presented in detail so as to be reproducible from this report. Regardless of assay method, salivary testosterone levels are modestly correlated with serum levels for males but not necessarily for females. Blood spot assay results were highly correlated with serum total and free testosterone for both males and females. Substitution of saliva assay results for serum values substantially underestimates known testosterone-behavior associations, and this effect is much more pronounced for females than for males. The findings have important implications for the use and potential misuse of noninvasive measures of testosterone, and with respect to statistical power, the probability of observing significant testosterone-behavior relationships.

AB - We rigorously evaluated gender differences in the measurement validity of salivary testosterone. Matched serum, saliva, and finger stick blood spot specimens were collected from 40 (20 males) young adults (aged 18-27 years). Saliva was assayed for testosterone by two independent (isotopic and non-isotopic) immunoassay methods. Serum was assayed by commercially available immunoassay kits for free and total testosterone. An immunoassay was developed for the measurement of testosterone in dried blood spots and is presented in detail so as to be reproducible from this report. Regardless of assay method, salivary testosterone levels are modestly correlated with serum levels for males but not necessarily for females. Blood spot assay results were highly correlated with serum total and free testosterone for both males and females. Substitution of saliva assay results for serum values substantially underestimates known testosterone-behavior associations, and this effect is much more pronounced for females than for males. The findings have important implications for the use and potential misuse of noninvasive measures of testosterone, and with respect to statistical power, the probability of observing significant testosterone-behavior relationships.

UR - http://www.scopus.com/inward/record.url?scp=0036672702&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036672702&partnerID=8YFLogxK

U2 - 10.1006/hbeh.2002.1798

DO - 10.1006/hbeh.2002.1798

M3 - Article

C2 - 12191648

AN - SCOPUS:0036672702

VL - 42

SP - 62

EP - 69

JO - Hormones and Behavior

JF - Hormones and Behavior

SN - 0018-506X

IS - 1

ER -