Geminiviral vectors based on bean yellow dwarf virus for production of vaccine antigens and monoclonal antibodies in plants

Qiang Chen, Junyun He, Waranyoo Phoolcharoen, Hugh Mason

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Expression of recombinant vaccine antigens and monoclonal antibodies using plant viral vectors has developed extensively during the past several years. The approach benefits from high yields of recombinant protein obtained within days after transient delivery of viral vectors to leaves of Nicotiana benthamiana, a tobacco relative. Modified viral genomes of both RNA and DNA viruses have been created. Geminiviruses such as bean yellow dwarf virus (BeYDV) have a small, single stranded DNA genome that replicates in the nucleus of an infected plant cell, using the cellular DNA synthesis apparatus and a virus-encoded replication initiator protein (Rep). BeYDV-derived expression vectors contain deletions of the viral genes encoding coat and movement proteins and insertion of an expression cassette for a protein of interest. Delivery of the geminiviral vector to leaf cells via Agrobacterium-mediated delivery produces very high levels of recombinant DNA that can act as a transcription template, yielding high levels of mRNA for the protein of interest. Several vaccine antigens, including Norwalk virus capsid protein and hepatitis B core antigen, were expressed using the BeYDV vector at levels up to 1 mg per g of leaf mass. BeYDV replicons can be stacked in the same vector molecule by linking them in tandem, which enables production of multi-subunit proteins like monoclonal antibody (mAb) heavy and light chains. The protective mAb 6D8 against Ebola virus was produced at 0.5 mg per g of leaf mass. Multi-replicon vectors could be conveniently used to produce protein complexes, e.g., virus-like particles that require two or more subunits.

Original languageEnglish (US)
Pages (from-to)331-338
Number of pages8
JournalHuman Vaccines
Volume7
Issue number3
DOIs
StatePublished - Mar 2011

Fingerprint

Vaccines
Monoclonal Antibodies
Viruses
Antigens
Replicon
Capsid Proteins
Tobacco
Geminiviridae
Ebolavirus
Hepatitis B Core Antigens
Agrobacterium
Synthetic Vaccines
Proteins
DNA Viruses
Recombinant DNA
Viral Genes
Viral Genome
Single-Stranded DNA
RNA Viruses
Protein Subunits

Keywords

  • Bean yellow dwarf virus
  • Geminiviral replicon
  • Monoclonal antibody
  • Plant-derived vaccine
  • Vaccine
  • Virus-like particle

ASJC Scopus subject areas

  • Immunology
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Geminiviral vectors based on bean yellow dwarf virus for production of vaccine antigens and monoclonal antibodies in plants. / Chen, Qiang; He, Junyun; Phoolcharoen, Waranyoo; Mason, Hugh.

In: Human Vaccines, Vol. 7, No. 3, 03.2011, p. 331-338.

Research output: Contribution to journalArticle

@article{71048070f71d49679871f24247ecaa3e,
title = "Geminiviral vectors based on bean yellow dwarf virus for production of vaccine antigens and monoclonal antibodies in plants",
abstract = "Expression of recombinant vaccine antigens and monoclonal antibodies using plant viral vectors has developed extensively during the past several years. The approach benefits from high yields of recombinant protein obtained within days after transient delivery of viral vectors to leaves of Nicotiana benthamiana, a tobacco relative. Modified viral genomes of both RNA and DNA viruses have been created. Geminiviruses such as bean yellow dwarf virus (BeYDV) have a small, single stranded DNA genome that replicates in the nucleus of an infected plant cell, using the cellular DNA synthesis apparatus and a virus-encoded replication initiator protein (Rep). BeYDV-derived expression vectors contain deletions of the viral genes encoding coat and movement proteins and insertion of an expression cassette for a protein of interest. Delivery of the geminiviral vector to leaf cells via Agrobacterium-mediated delivery produces very high levels of recombinant DNA that can act as a transcription template, yielding high levels of mRNA for the protein of interest. Several vaccine antigens, including Norwalk virus capsid protein and hepatitis B core antigen, were expressed using the BeYDV vector at levels up to 1 mg per g of leaf mass. BeYDV replicons can be stacked in the same vector molecule by linking them in tandem, which enables production of multi-subunit proteins like monoclonal antibody (mAb) heavy and light chains. The protective mAb 6D8 against Ebola virus was produced at 0.5 mg per g of leaf mass. Multi-replicon vectors could be conveniently used to produce protein complexes, e.g., virus-like particles that require two or more subunits.",
keywords = "Bean yellow dwarf virus, Geminiviral replicon, Monoclonal antibody, Plant-derived vaccine, Vaccine, Virus-like particle",
author = "Qiang Chen and Junyun He and Waranyoo Phoolcharoen and Hugh Mason",
year = "2011",
month = "3",
doi = "10.4161/hv.7.3.14262",
language = "English (US)",
volume = "7",
pages = "331--338",
journal = "Human Vaccines",
issn = "1554-8600",
publisher = "Landes Bioscience",
number = "3",

}

TY - JOUR

T1 - Geminiviral vectors based on bean yellow dwarf virus for production of vaccine antigens and monoclonal antibodies in plants

AU - Chen, Qiang

AU - He, Junyun

AU - Phoolcharoen, Waranyoo

AU - Mason, Hugh

PY - 2011/3

Y1 - 2011/3

N2 - Expression of recombinant vaccine antigens and monoclonal antibodies using plant viral vectors has developed extensively during the past several years. The approach benefits from high yields of recombinant protein obtained within days after transient delivery of viral vectors to leaves of Nicotiana benthamiana, a tobacco relative. Modified viral genomes of both RNA and DNA viruses have been created. Geminiviruses such as bean yellow dwarf virus (BeYDV) have a small, single stranded DNA genome that replicates in the nucleus of an infected plant cell, using the cellular DNA synthesis apparatus and a virus-encoded replication initiator protein (Rep). BeYDV-derived expression vectors contain deletions of the viral genes encoding coat and movement proteins and insertion of an expression cassette for a protein of interest. Delivery of the geminiviral vector to leaf cells via Agrobacterium-mediated delivery produces very high levels of recombinant DNA that can act as a transcription template, yielding high levels of mRNA for the protein of interest. Several vaccine antigens, including Norwalk virus capsid protein and hepatitis B core antigen, were expressed using the BeYDV vector at levels up to 1 mg per g of leaf mass. BeYDV replicons can be stacked in the same vector molecule by linking them in tandem, which enables production of multi-subunit proteins like monoclonal antibody (mAb) heavy and light chains. The protective mAb 6D8 against Ebola virus was produced at 0.5 mg per g of leaf mass. Multi-replicon vectors could be conveniently used to produce protein complexes, e.g., virus-like particles that require two or more subunits.

AB - Expression of recombinant vaccine antigens and monoclonal antibodies using plant viral vectors has developed extensively during the past several years. The approach benefits from high yields of recombinant protein obtained within days after transient delivery of viral vectors to leaves of Nicotiana benthamiana, a tobacco relative. Modified viral genomes of both RNA and DNA viruses have been created. Geminiviruses such as bean yellow dwarf virus (BeYDV) have a small, single stranded DNA genome that replicates in the nucleus of an infected plant cell, using the cellular DNA synthesis apparatus and a virus-encoded replication initiator protein (Rep). BeYDV-derived expression vectors contain deletions of the viral genes encoding coat and movement proteins and insertion of an expression cassette for a protein of interest. Delivery of the geminiviral vector to leaf cells via Agrobacterium-mediated delivery produces very high levels of recombinant DNA that can act as a transcription template, yielding high levels of mRNA for the protein of interest. Several vaccine antigens, including Norwalk virus capsid protein and hepatitis B core antigen, were expressed using the BeYDV vector at levels up to 1 mg per g of leaf mass. BeYDV replicons can be stacked in the same vector molecule by linking them in tandem, which enables production of multi-subunit proteins like monoclonal antibody (mAb) heavy and light chains. The protective mAb 6D8 against Ebola virus was produced at 0.5 mg per g of leaf mass. Multi-replicon vectors could be conveniently used to produce protein complexes, e.g., virus-like particles that require two or more subunits.

KW - Bean yellow dwarf virus

KW - Geminiviral replicon

KW - Monoclonal antibody

KW - Plant-derived vaccine

KW - Vaccine

KW - Virus-like particle

UR - http://www.scopus.com/inward/record.url?scp=79953675401&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79953675401&partnerID=8YFLogxK

U2 - 10.4161/hv.7.3.14262

DO - 10.4161/hv.7.3.14262

M3 - Article

C2 - 21358270

AN - SCOPUS:79953675401

VL - 7

SP - 331

EP - 338

JO - Human Vaccines

JF - Human Vaccines

SN - 1554-8600

IS - 3

ER -