gamma Ray induced deoxyribonucleic acid strand breaks. 3' Glycolate termini.

W. D. Henner, L. O. Rodriguez, S. M. Hecht, W. A. Haseltine

Research output: Contribution to journalArticle

282 Scopus citations

Abstract

Gamma irradiation of DNA in aqueous solution produces single strand breaks with two types of 3'-termini. These termini have been identified as a simple phosphoryl group and a glycolate moiety attached to the 3'-phosphoryl group via the 2"-OH of glycolic acid. Irradiation of 5'-end labeled poly(dG) produces compounds that comigrate with authentic 3',5'-dGDP and 2'-deoxyguanosine-3'-(phospho-2"-O-glycolic acid-5'-monophosphate by thin layer chromatography and polyacrylamide gel electrophoresis. Irradiation of poly(dGdC) followed by digestion to 3'-nucleotides produces 2'-deoxyguanosine-3'-(phospho-2"-O-glycolic acid). The presence of glycolate termini at some strand breaks may affect intracellular repair of these lesions.

Original languageEnglish (US)
Pages (from-to)711-713
Number of pages3
JournalJournal of Biological Chemistry
Volume258
Issue number2
StatePublished - Jan 25 1983
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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