gamma Ray induced deoxyribonucleic acid strand breaks. 3' Glycolate termini.

W. D. Henner, L. O. Rodriguez, Sidney Hecht, W. A. Haseltine

Research output: Contribution to journalArticle

281 Citations (Scopus)

Abstract

Gamma irradiation of DNA in aqueous solution produces single strand breaks with two types of 3'-termini. These termini have been identified as a simple phosphoryl group and a glycolate moiety attached to the 3'-phosphoryl group via the 2"-OH of glycolic acid. Irradiation of 5'-end labeled poly(dG) produces compounds that comigrate with authentic 3',5'-dGDP and 2'-deoxyguanosine-3'-(phospho-2"-O-glycolic acid-5'-monophosphate by thin layer chromatography and polyacrylamide gel electrophoresis. Irradiation of poly(dGdC) followed by digestion to 3'-nucleotides produces 2'-deoxyguanosine-3'-(phospho-2"-O-glycolic acid). The presence of glycolate termini at some strand breaks may affect intracellular repair of these lesions.

Original languageEnglish (US)
Pages (from-to)711-713
Number of pages3
JournalJournal of Biological Chemistry
Volume258
Issue number2
StatePublished - Jan 25 1983
Externally publishedYes

Fingerprint

glycolic acid
Gamma Rays
Gamma rays
Irradiation
DNA
Thin layer chromatography
Thin Layer Chromatography
Electrophoresis
Polyacrylamide Gel Electrophoresis
Digestion
Repair
Nucleotides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Henner, W. D., Rodriguez, L. O., Hecht, S., & Haseltine, W. A. (1983). gamma Ray induced deoxyribonucleic acid strand breaks. 3' Glycolate termini. Journal of Biological Chemistry, 258(2), 711-713.

gamma Ray induced deoxyribonucleic acid strand breaks. 3' Glycolate termini. / Henner, W. D.; Rodriguez, L. O.; Hecht, Sidney; Haseltine, W. A.

In: Journal of Biological Chemistry, Vol. 258, No. 2, 25.01.1983, p. 711-713.

Research output: Contribution to journalArticle

Henner, WD, Rodriguez, LO, Hecht, S & Haseltine, WA 1983, 'gamma Ray induced deoxyribonucleic acid strand breaks. 3' Glycolate termini.', Journal of Biological Chemistry, vol. 258, no. 2, pp. 711-713.
Henner, W. D. ; Rodriguez, L. O. ; Hecht, Sidney ; Haseltine, W. A. / gamma Ray induced deoxyribonucleic acid strand breaks. 3' Glycolate termini. In: Journal of Biological Chemistry. 1983 ; Vol. 258, No. 2. pp. 711-713.
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