@article{90cc15e3d8ac46f38c11c66827b6a32d,
title = "Fusion partner toolchest for the stabilization and crystallization of G protein-coupled receptors",
abstract = "Structural studies of human G protein-coupled receptors (GPCRs) have recently been accelerated through the use of a fusion partner that was inserted into the third intracellular loop. Using chimeras of the human β2-adrenergic and human A2A adenosine receptors, we present the methodology and data for the initial selection of an expanded set of fusion partners for crystallizing GPCRs. In particular, use of the thermostabilized apocytochrome b562RIL as a fusion partner displays certain advantages over previously utilized fusion proteins, resulting in a significant improvement in stability and structure of GPCR-fusion constructs.",
author = "Eugene Chun and Thompson, {Aaron A.} and Wei Liu and Roth, {Christopher B.} and Griffith, {Mark T.} and Vsevolod Katritch and Joshua Kunken and Fei Xu and Vadim Cherezov and Hanson, {Michael A.} and Stevens, {Raymond C.}",
note = "Funding Information: This work was supported by NIH Common Fund in Structural Biology Grant P50 GM073197 and by the NCRR and NIGMS Grant R21 GM103972 for technology development. The authors thank J. Velasquez for help on molecular biology; T. Trinh, K. Allin, and M. Chu for help on baculovirus expression; A. Walker for assistance with manuscript preparation; L. Heitman and A. IJzerman for critical review of the manuscript. R.C.S. is a founder and on the Board of Directors of Receptos, a GPCR structure-based drug discovery company. ",
year = "2012",
month = jun,
day = "6",
doi = "10.1016/j.str.2012.04.010",
language = "English (US)",
volume = "20",
pages = "967--976",
journal = "Structure with Folding & design",
issn = "0969-2126",
publisher = "Cell Press",
number = "6",
}