Fusion partner toolchest for the stabilization and crystallization of G protein-coupled receptors

Eugene Chun, Aaron A. Thompson, Wei Liu, Christopher B. Roth, Mark T. Griffith, Vsevolod Katritch, Joshua Kunken, Fei Xu, Vadim Cherezov, Michael A. Hanson, Raymond C. Stevens

Research output: Contribution to journalArticle

180 Citations (Scopus)

Abstract

Structural studies of human G protein-coupled receptors (GPCRs) have recently been accelerated through the use of a fusion partner that was inserted into the third intracellular loop. Using chimeras of the human β2-adrenergic and human A2A adenosine receptors, we present the methodology and data for the initial selection of an expanded set of fusion partners for crystallizing GPCRs. In particular, use of the thermostabilized apocytochrome b562RIL as a fusion partner displays certain advantages over previously utilized fusion proteins, resulting in a significant improvement in stability and structure of GPCR-fusion constructs.

Original languageEnglish (US)
Pages (from-to)967-976
Number of pages10
JournalStructure
Volume20
Issue number6
DOIs
StatePublished - Jun 6 2012
Externally publishedYes

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G-Protein-Coupled Receptors
Crystallization
Adenosine A2A Receptors
Adrenergic Agents
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

Chun, E., Thompson, A. A., Liu, W., Roth, C. B., Griffith, M. T., Katritch, V., ... Stevens, R. C. (2012). Fusion partner toolchest for the stabilization and crystallization of G protein-coupled receptors. Structure, 20(6), 967-976. https://doi.org/10.1016/j.str.2012.04.010

Fusion partner toolchest for the stabilization and crystallization of G protein-coupled receptors. / Chun, Eugene; Thompson, Aaron A.; Liu, Wei; Roth, Christopher B.; Griffith, Mark T.; Katritch, Vsevolod; Kunken, Joshua; Xu, Fei; Cherezov, Vadim; Hanson, Michael A.; Stevens, Raymond C.

In: Structure, Vol. 20, No. 6, 06.06.2012, p. 967-976.

Research output: Contribution to journalArticle

Chun, E, Thompson, AA, Liu, W, Roth, CB, Griffith, MT, Katritch, V, Kunken, J, Xu, F, Cherezov, V, Hanson, MA & Stevens, RC 2012, 'Fusion partner toolchest for the stabilization and crystallization of G protein-coupled receptors', Structure, vol. 20, no. 6, pp. 967-976. https://doi.org/10.1016/j.str.2012.04.010
Chun, Eugene ; Thompson, Aaron A. ; Liu, Wei ; Roth, Christopher B. ; Griffith, Mark T. ; Katritch, Vsevolod ; Kunken, Joshua ; Xu, Fei ; Cherezov, Vadim ; Hanson, Michael A. ; Stevens, Raymond C. / Fusion partner toolchest for the stabilization and crystallization of G protein-coupled receptors. In: Structure. 2012 ; Vol. 20, No. 6. pp. 967-976.
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