Fushi tarazu protein expression in the cellular blastoderm of Drosophila detected using a novel imaging technique

T. L. Karr, T. B. Kornberg

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The fushi tarazu (ftz) gene is essential for segmentation of the Drosophila embryo. This requirement is reflected at the cellular blastoderm stage of embryogenesis by seven transverse stripes of ftz expression. These stripes correspond to the missing segments of ftz mutant embryos. We describe here novel intermediate patterns of ftz protein expression which were detected in younger embryos by using anti-ftz antibodies and a sensitive fluorescence/immunoperoxidase technique ('filtered fluorescence imaging', FFI). Striped patterns of ftz protein evolved continuously, and the different stripes appeared in an ordered sequence, involving both anterior-posterior (A/P) and dorsal-ventral (D/V) progressions. Comparison of these patterns of ftz protein with those of ftz RNA suggests that these novel aspects of the patterning process involve post-transcriptional regulation in addition to the transcriptional control known to be involved in expression of this gene.

Original languageEnglish (US)
Pages (from-to)95-103
Number of pages9
JournalDevelopment
Volume106
Issue number1
StatePublished - Jan 1 1989
Externally publishedYes

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Fushi Tarazu Transcription Factors
Blastoderm
embryo
Drosophila
Embryonic Structures
protein
fluorescence
gene
Optical Imaging
Essential Genes
Immunoenzyme Techniques
segmentation
antibody
Embryonic Development
RNA
Fluorescence
Gene Expression
regulation
Antibodies

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Cite this

Fushi tarazu protein expression in the cellular blastoderm of Drosophila detected using a novel imaging technique. / Karr, T. L.; Kornberg, T. B.

In: Development, Vol. 106, No. 1, 01.01.1989, p. 95-103.

Research output: Contribution to journalArticle

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