Functional delivery of a membrane protein into oocyte membranes using bicelles

Congbao Kang, Carlos G. Vanoye, Richard C. Welch, Wade D. Van Horn, Charles R. Sanders

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Voltage-gated potassium channel modulatory membrane proteinKCNE3 was overexpressed and purified into both micelles and bicelles. Remarkably, microinjection of KCNE3 in bicelles into Xenopus oocytes resulted in functional co-assembly with the human KCNQ1 channel expressed therein. Microinjection of LMPC micelles containing KCNE3 did not result in channel modulation, indicating that bicelles sometimes succeed at delivering a membrane protein into a cellular membrane when classical micelles fail. Backbone NMR resonance assignments were completed for KCNE3 in both bicelles and LMPC, indicating that the secondary structure distribution in KCNE3's N-terminus and transmembrane domains exhibits only modest differences from that of KCNE1, even though these KCNE family members have very different effects on KCNQ1 channel function.

Original languageEnglish (US)
Pages (from-to)653-655
Number of pages3
JournalBiochemistry
Volume49
Issue number4
DOIs
StatePublished - Feb 2 2010
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Functional delivery of a membrane protein into oocyte membranes using bicelles'. Together they form a unique fingerprint.

  • Cite this