Functional Characterization of Mutant Strains of the Cyanobacterium Synechocystis sp. PCC 6803 Lacking Short Domains within the Large, Lumen-Exposed Loop of the Chlorophyll Protein CP47 in Photosystem II

Hermann M. Gleiter, Elisabeth Haag, Jian Ren Shen, Julian J. Eaton-Rye, Yorinao Inoue, Willem Vermaas, Gernot Renger

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Abstract

Several autotrophic mutant strains of Synechocystis sp. PCC 6803 carrying short deletions or a single-site mutation within the large, lumen-exposed loop (loop E) of the chlorophyll a-binding photosytem II core protein, CP47, are analyzed for their functional properties by measuring the flash-induced pattern of thermoluminescence, oxygen yield, and fluorescence quantum yield. A physiological and biochemical characterization of these mutant strains has been given in two previous reports [Eaton-Rye, J. J., & Vermaas, W. F. J. (1991) Plant Mol. Biol. 17, 1165-1177; Haag, E., Eaton-Rye, J. J., Renger, G., & Vermaas, S. F. J. (1993) Biochemistry 32, 4444-4454]. The results of the present study show that deletion of charged and conserved amino acids in a region roughly located between residues 370 and 390 decreases the binding affinity of the extrinsic PS II-O protein to photosystem II. Marked differences with PSII-O deletion mutants are observed with respect to Ca2+ requirement and the flash-induced pattern of oxygen evolution. Under conditions where a sufficient light activation is provided, the psbB mutants assayed in this study reveal normal S-state parameters and lifetimes. The results bear two basic implications: (i) the manganese involved in water oxidation can still be bound in a functionally normal or only slightly distorted manner, and (ii) the binding of the extrinsic PS II-O protein to photosystem II is impaired in mutants carrying a deletion in the domain between residues 370 and 390, but the presence of the PS II-O protein is still of functional relevance for the PS II complex, e.g., for maintenance of a high-affinity binding site for Ca2+ and/or involvement during the process of photoactivation.

Original languageEnglish (US)
Pages (from-to)12063-12071
Number of pages9
JournalBiochemistry
Volume33
Issue number40
DOIs
StatePublished - Oct 1 1994

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ASJC Scopus subject areas

  • Biochemistry

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