Function of sll1906, a member of the bacteriochlorophyll delivery family, in the cyanobacterium synechocystis sp. PCC 6803

Cheng I.Daniel Yao, Willem Vermaas

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A deletion mutation was introduced into the sll1906 gene in the cyanobacterium Synechocystis sp. PCC 6803 to examine the function of Sll1906, a member of the putative “bacteriochlorophyll delivery” protein family. The Sll1906 sequence contains possible chlorophyll-binding sites. The pigment profile indicated that the chlorophyll and carotenoids contents were not altered in the mutant, and no chlorophyll precursors accumulated. According to the oxygen evolution and 77 K fluorescence emission spectra, the PSII activity and PSII/PSI ratio remained the same upon deletion of the gene. The sll1906 deletion was also introduced into the chlL background mutant strain, in which chlorophyll is synthesized in the light only. When grown in light-activated heterotrophic growth (LAHG) conditions, the rate of chlorophyll degradation in the chlL sll1906mutant was similar to that in the chlL background strain. When cells were returned to continuous illumination after a week of growth under LAHG conditions, both the rate of chlorophyll synthesis and chlorophyll-dependent photosystem biogenesis were monitored. The deletion of the sll1906 gene affected neither. Although the sll1906 deletion did not affect chlorophyll degradation/biosynthesis and photosystem assembly, Sll1906 could still be involved in these processes as other pathways may compensate for the absence of Sll1906.

Original languageEnglish (US)
Title of host publicationAdvanced Topics in Science and Technology in China
PublisherSpringer
Pages409-414
Number of pages6
DOIs
StatePublished - Jan 1 2013

Publication series

NameAdvanced Topics in Science and Technology in China
ISSN (Print)1995-6819
ISSN (Electronic)1995-6827

Fingerprint

Bacteriochlorophylls
Chlorophyll
Genes
Degradation
Cyanobacteria
Biosynthesis
Binding sites
Carotenoids
Pigments
Lighting
Fluorescence
Binding Sites
Oxygen
Proteins

Keywords

  • Chlorophyll
  • PucC
  • sll1906
  • Synechocystis

ASJC Scopus subject areas

  • Engineering(all)
  • Chemical Engineering(all)
  • General

Cite this

Yao, C. I. D., & Vermaas, W. (2013). Function of sll1906, a member of the bacteriochlorophyll delivery family, in the cyanobacterium synechocystis sp. PCC 6803. In Advanced Topics in Science and Technology in China (pp. 409-414). (Advanced Topics in Science and Technology in China). Springer. https://doi.org/10.1007/978-3-642-32034-7_85

Function of sll1906, a member of the bacteriochlorophyll delivery family, in the cyanobacterium synechocystis sp. PCC 6803. / Yao, Cheng I.Daniel; Vermaas, Willem.

Advanced Topics in Science and Technology in China. Springer, 2013. p. 409-414 (Advanced Topics in Science and Technology in China).

Research output: Chapter in Book/Report/Conference proceedingChapter

Yao, CID & Vermaas, W 2013, Function of sll1906, a member of the bacteriochlorophyll delivery family, in the cyanobacterium synechocystis sp. PCC 6803. in Advanced Topics in Science and Technology in China. Advanced Topics in Science and Technology in China, Springer, pp. 409-414. https://doi.org/10.1007/978-3-642-32034-7_85
Yao CID, Vermaas W. Function of sll1906, a member of the bacteriochlorophyll delivery family, in the cyanobacterium synechocystis sp. PCC 6803. In Advanced Topics in Science and Technology in China. Springer. 2013. p. 409-414. (Advanced Topics in Science and Technology in China). https://doi.org/10.1007/978-3-642-32034-7_85
Yao, Cheng I.Daniel ; Vermaas, Willem. / Function of sll1906, a member of the bacteriochlorophyll delivery family, in the cyanobacterium synechocystis sp. PCC 6803. Advanced Topics in Science and Technology in China. Springer, 2013. pp. 409-414 (Advanced Topics in Science and Technology in China).
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