Function and subnuclear distribution of Rpp21, a protein subunit of the human ribonucleoprotein ribonuclease P

Nayef Jarrous, Robert Reiner, Donna Wesolowski, Hagit Mann, Cecilia Guerrier-Takada, Sidney Altman

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Rpp21, a protein subunit of human nuclear ribonuclease P (RNase P) was cloned by virtue of its homology with Rpr2p, an essential subunit of Saccharomyces cerevisiae nuclear RNase P. Rpp21 is encoded by a gene that resides in the class I gene cluster of the major histocompatibility complex, is associated with highly purified RNase P, and binds precursor tRNA. Rpp21 is predominantly localized in the nucleoplasm but is also observed in nucleoli and Cajal bodies when expressed at high levels. Intron retention and splice-site selection in Rpp21 precursor mRNA regulate the intranuclear distribution of the protein products and their association with the RNase P holoenzyme. Our study reveals that dynamic nuclear structures that include nucleoli, the perinucleolar compartment and Cajal bodies are all involved in the production and assembly of human RNase P.

Original languageEnglish (US)
Pages (from-to)1153-1164
Number of pages12
JournalRNA
Volume7
Issue number8
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Ribonuclease P
Ribonucleoproteins
Protein Subunits
RNA Precursors
MHC Class I Genes
Holoenzymes
Multigene Family
Major Histocompatibility Complex
Introns
Saccharomyces cerevisiae
Genes
Proteins

Keywords

  • Cajal bodies
  • Nucleolus
  • Precursor tRNA
  • Ribonuclease MRP
  • Ribonuclease P

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Function and subnuclear distribution of Rpp21, a protein subunit of the human ribonucleoprotein ribonuclease P. / Jarrous, Nayef; Reiner, Robert; Wesolowski, Donna; Mann, Hagit; Guerrier-Takada, Cecilia; Altman, Sidney.

In: RNA, Vol. 7, No. 8, 2001, p. 1153-1164.

Research output: Contribution to journalArticle

Jarrous, Nayef ; Reiner, Robert ; Wesolowski, Donna ; Mann, Hagit ; Guerrier-Takada, Cecilia ; Altman, Sidney. / Function and subnuclear distribution of Rpp21, a protein subunit of the human ribonucleoprotein ribonuclease P. In: RNA. 2001 ; Vol. 7, No. 8. pp. 1153-1164.
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AU - Jarrous, Nayef

AU - Reiner, Robert

AU - Wesolowski, Donna

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AU - Guerrier-Takada, Cecilia

AU - Altman, Sidney

PY - 2001

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